上皮黏附因子與Oct4或Klf4可以透過活化轉錄激活蛋白3與低氧誘導蛋白2a生成誘導性多能幹細胞

管奕奕

doi:10.6342/NTU201700408

山西大学，您好！

感谢采购，人社期刊库｜人社论文库，期间：2025/01/01~2025/12/31

感谢试用，科学期刊库｜科学论文库，期间：2025/01/21~2025/03/31

浏览高级检索

关闭
检索检索高级检索出版物检索
浏览期刊主题浏览核心期刊浏览期刊拼音浏览

关闭

查询符号(半角)	查询符号意义说明
空格	表示为「AND」两个查询词之交集
双引号 ( " " )	片语以双引号标示开始及结束，而且只寻找出现顺序相同的字词，例： " image process "
?	表示一个字母切截，输入两个?表两个字母，依此类推，例：输入「Appl?」，查得结果应为appl e , appl y … ( 常用于英文字查询 )
*	表示不限字母切截，由0~n. 例：输入「appl*」，查得结果应为appl e , appl es , appl y , appl ied , appl ication … ( 常用于英文字查询 )
AND、OR、NOT	布尔逻辑组合关键字，用来扩大或缩小查询范围的技巧。 (1) AND ：缩小查询范围 (2) OR ：扩大查询范围(3) NOT：排除不相关的范围

DOI 是数位物件识别码（ D igital O bject I dentifier ）的简称，
为物件在网路上的唯一识别码，可用于永久连结并引用目标物件。

使用DOI作为永久连结

每个DOI号前面加上「 http://dx.doi.org/ 」便成为永久网址。
如以DOI号为 10.5297/ser.1201.002 的文献为例，此文献的永久连结便是： http://dx.doi.org/ 10.5297/ser.1201.002 。
日后不论出版单位如何更动此文献位置，永久连结所指向的位置皆会即时更新，不再错失重要的研究。

引用含有DOI的文献

有DOI的文献在引用时皆应同时引用DOI。若使用APA、Chicago以外未规范DOI的引用格式，可引用DOI永久连结。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。

书目管理工具

相关连结

问题回报

全文下载

上皮黏附因子与Oct4或Klf4可以透过活化转录激活蛋白3与低氧诱导蛋白2a生成诱导性多能干细胞

EpCAM/EpEX and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2a

管奕奕 , 博士　　导师：吴汉忠　　

英文 DOI： 10.6342/NTU201700408

上皮细胞粘附分子；诱导型多能性干细胞；山中伸弥因子；重编程； EpEX ； EpCAM ； induced pluripotent stem cells ； reprogramming

0 个人觉得这篇文章赞

摘要 │ 参考文献 (199) │ 国际计量

摘要〈TOP〉

并列摘要〈TOP〉

Induced pluripotent stem cells (iPSCs) are generated from somatic cells, and have pluripotency and self-renewal ability. iPSCs holds great promising for its multiple application including drug screening, disease remolding, and even the possibility of autologous transplantation. However, until now, most of the iPSCs are generated by the transduction of four Yamanaka factors. The epithelial cell adhesion molecule (EpCAM), a type I transmembrane glycoprotein, is a potent stem cell surface marker. The intracellular domain of EpCAM (EpICD) upregulates reprogramming genes, including Oct4 (O), Sox2 (S), Klf4 (K), c-Myc (M), and Nanog (N), through association with their promoters. Therefore, we are prompted to investigate the functional roles of EpCAM in reprogramming of fibroblasts into iPSCs and the underlying molecular mechanisms. Here, we found that OSKM with EpCAM/EpEX increased the iPSC formation up to 2.4-fold compared to OSKM iPSCs. Interestingly, single Yamanaka factor, Oct4 or Klf4, but not Sox2, can successfully form iPSCs with the presence of EpCAM and EpEX. The characteristics of iPSCs generated by one Yamanaka factor with EpCAM (E) + EpEX were further confirmed by in vitro and in vivo assays. Moreover, these iPSCs could differentiate into three germ layers in teratoma and contribute to germline transmission in chimera mice. Furthermore, we demonstrated that EpEX and EpCAM can initiate reprogramming via activation of STAT3 signaling and nuclear-translocation of HIF2a, thereby providing a positive feedback loops for pluripotent circuit. Through our study, we present a new way of triggering reprogramming by membrane protein and soluble factor with single Yamanaka factor, and highlight the novel finding of EpCAM/EpEX-STAT3-HIF2a signals. Understanding these signals is of great interests for enhancing our fundamental knowledge of EpCAM in reprogramming process and establishing a new reprogramming strategy by delivery of membrane protein and soluble factors.

参考文献 ( 199 ) 〈TOP〉

Aasen, T., Raya, A., Barrero, M.J., Garreta, E., Consiglio, A., Gonzalez, F., Vassena, R., Bilic, J., Pekarik, V., Tiscornia, G., et al. (2008). Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nature biotechnology 26, 1276-1284.
连结：
Abramovich, A., Muradian, K.K., and Fraifeld, V.E. (2008). Have we reached the point for in vivo rejuvenation? Rejuvenation research 11, 489-492.
连结：
Agger, K., Cloos, P.A., Christensen, J., Pasini, D., Rose, S., Rappsilber, J., Issaeva, I., Canaani, E., Salcini, A.E., and Helin, K. (2007). UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature 449, 731-734.
连结：
Amoury, M., Kolberg, K., Pham, A.T., Hristodorov, D., Mladenov, R., Di Fiore, S., Helfrich, W., Kiessling, F., Fischer, R., Pardo, A., et al. (2016). Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model. Cancer letters 372, 201-209.
连结：
Ancot, F., Foveau, B., Lefebvre, J., Leroy, C., and Tulasne, D. (2009). Proteolytic cleavages give receptor tyrosine kinases the gift of ubiquity. Oncogene 28, 2185-2195.
连结：

国际计量

〈TOP〉

E-mail ：

文章公开取用时，将寄通知信至您填写的信箱地址

E-mail ：

使用DOI作为永久连结

引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。