微型核糖核酸2111及169對圓葉菸草(Nicotiana benthamiana)在缺磷和乾旱逆境耐受性上所扮演的角色

黃莉詠

doi:10.6342/NTU201904219

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微型核糖核酸2111及169对圆叶菸草(Nicotiana benthamiana)在缺磷和乾旱逆境耐受性上所扮演的角色

The role of nbe-miR2111 and nbe-miR169 in the tolerance to phosphorus deficiency and drought stress in tobacco (Nicotiana benthamiana)

黄莉咏 , 硕士　　导师：林淑怡　　

繁体中文 DOI： 10.6342/NTU201904219

微型核糖核酸；磷；乾旱；圆叶菸草； microRNA ； phosphorus ； drought ； Nicotiana benthamiana ； Nicotiana benthamiana ；

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摘要 │ 参考文献 (108)

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In recent years, many researches have pointed out that plant microRNAs (miRNAs) play key roles in abiotic stresses tolerance. Among them, miR2111 and miR169 were regulates by the phosphorus deficiency and drought stress in previous studies. To explore the roles of nbe-miR2111 and nbe-miR169 in response to phosphorus deficiency and drought stress in tobacco (Nicotiana benthamiana), we generated transgenic tobacco which overexpress nbe-miR2111 (PCoYMV::nbe-miR2111) and nbe-miR169 (P35S::nbe-miR169). Wild-type (WT) tobacco and three transgenic lines were subjected to phosphorus deficiency and drought treatment at the seedling and adult stage. Most of the measurement items showed no consistent results or no significant difference between PCoYMV::nbe-miR2111 and P35S::nbe-miR169 with WT seedlings and adult plants under three different phosphorus concentrations: 500 μM (normal phosphorus), 50 μM (low phosphorus) and 0 μM (absence of phosphorus). Under the treatment of absence of phosphorus, the lower leaves of P35S::nbe-miR169 transgenic plants first appeared necrosis. However, after re-supply of phosphorus fertilizer, the fresh weight of P35S::nbe-miR169 transgenic plants was significantly higher than that of WT, suggesting that the transgenic plants may have better recovery ability than WT by sacrificing the lower leaves under treatment of absence of phosphorus. After drought stress and re-watering, the P35S::nbe-miR169 transgenic plants have better resilience, such as leaves no longer droop, higher shoot fresh weight, higher relative water content and higher antioxidant enzyme activities- including higher ascorbate peroxidase (APX) and catalase (CAT) activity. Stress indicators, such relativity electrolyte leakage, hydrogen peroxide (H2O2) concentration, malondialdehyde (MDA) concentrations, proline content and damaged cells was lower than WT. Within transgenic plants, line 12-3 is the best and then is line 3-5, line 1-1 only better than WT.

参考文献 ( 108 ) 〈TOP〉

Abid, M., S. Ali, L.K. Qi, R. Zahoor, Z.W. Tian, D. Jiang, J.L. Snider, and T.B. Dai. 2018. Physiological and biochemical changes during drought and recovery periods at tillering and jointing stages in wheat (Triticum aestivum L.). Sci. Rpt. 8:4615.
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引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。