發展具有比例螢光輸出酸性應答型前藥奈米粒子應用於藥物制放

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doi:10.6342/NTU201704279

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发展具有比例萤光输出酸性应答型前药奈米粒子应用於药物制放

Development of Acid-Responsive Prodrugs Nanoparticles with Fluorescence Ratiometric Readout for Application in Drug Delivery

刘力玮 , 硕士　　导师：陈昭岑　　

繁体中文 DOI： 10.6342/NTU201704279

酸刺激回应聚碳酸酯；自我组装；比例萤光输出； 3-羟基黄酮；萤光共振能量转移；药物传递； acid-responsive polycarbonates ； self-assembly ； fluorescence ratiometric readout ； 3-hydroxyflavone ； FRET ； drug delivery

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摘要 │ 参考文献 (107) │ 国际计量

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Poor bioavailability of clinically used chemotherapy drugs such as Camptothecin (CPT) and Paclitaxel (PTX) having low water solubility and the limited dose at the target sites has been a long standing problem for effective therapy. Polymeric drug delivery system, which can improve the water solubility, prolonging circulation time, increasing the amount of drug at the target sites, is a promising carrier to alleviate the problem encountered in the clinic. Utilizing unique phenomena such as hypoxia, higher temperature, acidic milieu, and overexpressing specific biomarkers of the cancer cells, which proliferate fast and metabolize actively, to design theranostic agents for specifically targeting cancer cells and minimizing the adverse effects on the normal cells is a highly fervent pursuit in recent years.	Propelled by these motives, two types of acid-responsive polymeric nanoparticles consisting of biodegradable diblock polycarbonate, hydrazine-linked Camptothecin (CPT)/Paclitaxel (PTX), and fluorophores to serve as theranostic agents for effectively delivering Camptothecin (CPT)/Paclitaxel (PTX) and possible imaging in the cells are designed and fabricated. In response to the low pH milieu (e.g. in lysosomes), the hydrazone bonds are hydrolyzed turning the hydrophobic part into a hydrophilic hydrazine moieties in the 3HF-hzPTXPC design. Subsequently the micelle structure would swell or disassemble with the concomitant release of the loaded drug and the fluorescence color of 3-HF changes from green to blue. In the DOX-hzCPTPC design, hydrazone bonds were used to covalently link the fluorescent drug (CPT) as a hydrophobic part and Doxorubicin (DOX) was encapsulated inside the micelles as well. CPT and DOX are both in the confined space facilitating the FRET. Once the micelles swell or disassemble, CPT and DOX are well separated resulting in the low FRET. Synergistically therapeutic effects are expected to achieve.
The morphology and the fluorescence behaviors of the micelle were studied by DLS, TEM and fluorescence microscopy respectively. In the case of 3HF-hzPTXPC, the results indicated that the micelles increase 1.3 fold in diameter with ratiometric fluorescence readout under acid milieu (pH 5.0). In addition, there were no changes in the size and fluorescence color of the micelles at physiological pH value (pH 7.4). The drug release experiment of micelles was conducted by HPLC analysis. PTX was released 2.1 fold at acid milieu than at physiological pH value. Furthermore, cell uptake study confirmed that the micelles are uptake by the cells. In vitro cytotoxicity analysis showed that the micelles can use fewer drugs to kill the cancer cells than free PTX. Collectively, 3HF-hzPTXPC can be potentially served as theranostic nanoparticle. In the case of DOX-hzCPTPC, the micelles increased 1.4 fold in its diameter under acid milieu. However, there were fluorescence color changes no matter under acid milieu or pH 7.4 due to the ring opening of CPT resulting in the low FRET at pH 7.4. As a result, the design of DOX-hzCPTPC micelles is needed to reconstruct.

参考文献 ( 107 ) 〈TOP〉

2. K. M. Huh, S. C. Lee, Y. W. Cho, J. Lee, J. H. Jeong, K. Park J. Control. Release 2005, 101, 59-68.
连结：
4. F. Danhier, O. Feron, V. Préat J. Control. Release 2010, 148, 135-146.
连结：
5. G. Bergers, L. E. Benjamin Nat. Rev. Cancer 2003, 3, 401-410.
连结：
10. Z. Ge, S. Liu Chem. Soc. Rev. 2013, 42, 7289-7325.
连结：
12. T. L. Andresen, D. H. Thompson, T. Kaasgaard Mol. Membr. Biol. 2010, 27, 353-363.
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使用DOI作为永久连结

引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。