體外和體內研究蓮蓬萃取物抵抗乙醯胺基苯酚誘導肝損傷之保護作用

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体外和体内研究莲蓬萃取物抵抗乙醯胺基苯酚诱导肝损伤之保护作用

In vitro and in vivo protective effect of Lotus seedpod extract against acetaminophen-induced liver injury

黄筱尹 , 硕士　　导师：陈璟贤　　

繁体中文

乙醯胺基苯酚；肝损伤；莲蓬萃取物；表没食子儿茶素；细胞凋亡；发炎； acetaminophen ； liver injury ； lotus seedpod extract ； epigallocatechin ； apoptosis ； inflammation

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摘要 │ 参考文献 (94) │ 国际计量

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Acetaminophen (APAP) is one of the most widely used analgesic and antipyretic drug in the world. APAP overdose will lead to severe liver injury and have potential to result in acute liver failure. Lotus seedpod, a traditional herbal, is rich in polyphenol and has been shown to possess antioxidant, radioprotective and anti-cancer activities. However, there were no significant reports on the hepatoprotective effect of LSE. In this study, we examined the hepatoprotective role of lotus seedpod extracts (LSE) in vitro and in vivo. Firstly, LSE or its main compound epigallocatechin (EGC) dose-dependently improved the survival of human hepatocyte HepG2 cells from APAP-induced loss of viability. LSE or EGC showed potential in reducing APAP-induced occurrence of apoptosis confirmed by morphological and biochemical features, including an increase in the distribution of hypodiploid phase, apoptotic bodies formation and caspases activation. Molecular data showed that antiapoptotic effects of LSE or EGC might be mediated via intrinsic, extrinsic and ASK 1/JNK apoptotic signaling pathways. Further data showed that LSE inhibited the APAP-induced the protein expression of iNOS. In vivo study, the BALB/c mice were supplemented with or without LSE (1% and 2%) during the 9-week treatment period in the presence or absence of APAP (i.p.; 400 mg/kg) twice per week. Our investigation demonstrated that LSE treatments significantly decreased the serum levels of the hepatic enzyme markers GOT and GPT, and triglyceride (TG) induced by APAP. LSE also inhibited the serum levels of inflammatory cytokines (IL-6 and IL-1β) during APAP treatment. LSE at 1% significantly restored the decrease in glutathione (GSH) content and elevated the levels of antioxidant enzymes, including catalase and glutathione reductase (GRd), in the liver. Western blotting data demonstrated that LSE and N-acetylcysteine (NAC) inhibited the expression of caspase -3, -8, -9 in APAP-induced liver injury. Our data imply that LSE reduced APAP-induced hepatocytes apoptosis, and these findings may open interesting perspectives to the strategy in treatment of liver injury.

参考文献 ( 94 ) 〈TOP〉

1. Fainboim L, Cherñavsky A, Paladino N, Flores AC, Arruvito L. Cytokines and chronic liver disease. Cytokine & growth factor reviews. 2007; 18:143-157.
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2. Sherlock S and Dooley J. Diseases of the liver and biliary system. 2008: John Wiley & Sons.
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4. Thomson AW and Knolle PA. Antigen-presenting cell function in the tolerogenic liver environment. Nature Reviews Immunology. 2010; 10: 753-766.
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5. Mitra V and Metcalf J. Metabolic functions of the liver. Anaesthesia & Intensive Care Medicine. 2009; 10: 334-335.
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8. Lu Y. and Cederbaum AI. CYP2E1 and oxidative liver injury by alcohol. Free Radical Biology and Medicine. 2008; 44: 723-738.
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使用DOI作为永久连结

引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。