利用固態發酵生產不同芽孢桿菌菌株

康智雄

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利用固态发酵生产不同芽孢杆菌菌株

Solid-state fermentation of various Bacillus species

康智雄 , 硕士　　导师：郭嘉信　　

英文

地衣芽孢杆菌；枯草芽孢杆菌；纳豆芽孢杆菌；固态发酵； Bacillus licheniformis ； Bacillus subtilis ； Bacillus subtilis var. natto ； Solid state fermentation

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摘要 │ 参考文献 (93) │ 国际计量

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Nowadays, antibiotics are widely used in livestock for increasing feed conversion efficacy and reducing costs. However, abuses of antibiotics have caused water and environment pollutions, as well as development of drug resistant pathogens, which become a major threat of human health. Researching alternative methods to reduce the use of antibiotics has become an important task or challenge in animal husbandry. Using probiotics to replace antibiotics in feeds is one of fensible strategies. People have drawn a great attention to the use of Bacillus spp. in animal feed. The present study investigated solid-state fermentation of Bacillus licheniformis, B. subtilis and B. subtilis var. natto using different agricultural by-products as fermentation substrates to make high cell density products as animal feed supplements. The investigation was initially studied on a lab scale and later was scaled up to a pilot production. The experimental results showed that rice husk to soybean meal on 2:1 ratio is suitable for use as medium formulation for cultivation of B. licheniformis, B. subtilis and B. subtilis var. natto. Sterilization time of 3 hours was required to kill all the heat resistant spores in the fermentation substrates. Additionally, glucose has to be sterilized separately from other ingredients in order to avoid Mallard reaction, which was found to inhibit the growth of Bacilli in this study. The optimized medium formulation and solid state fermentation process can produce fermentation products containing bacteria spores up to 10 to the power of 10 CFU/g after 3 days fermentation, which is satisfactory for the commercial production purpose.

参考文献 ( 93 ) 〈TOP〉

Adams, T. T., M. A. Eiteman, & B. M. Hanel. (2002). Solid state fermentation of broiler litter for production of biocontrol agents. Bioresource technology, 82(1), 33-41.
连结：
Alexopoulos, C., I. E. Georgoulakis, A. Tzivara, S. Kritas, A. Siochu, & S. Kyriakis. (2004). Field evaluation of the efficacy of a probiotic containing Bacillus licheniformis and Bacillus subtilis spores, on the health status and performance of sows and their litters. Journal of Animal Physiology and Animal Nutrition, 88(11‐12), 381-392.
连结：
Balasubramanian, B., T. Li, & I. H. Kim. (2016). Effects of supplementing growing-finishing pig diets with Bacillus spp. probiotic on growth performance and meat-carcass grade qualitytraits. Revista Brasileira de Zootecnia, 45(3), 93-100.
连结：
Boukhris, I., A. Farhat-Khemakhem, M. Blibech, K. Bouchaala, & H. Chouayekh. (2015). Characterization of an extremely salt-tolerant and thermostable phytase from Bacillus amyloliquefaciens US573. International journal of biological macromolecules, 80, 581-587.
连结：
Cavazzoni, V., A. Adami, & C. Castrovilli. (1998). Performance of broiler chickens supplemented with Bacillus coagulans as probiotic. British poultry science, 39(4), 526-529.
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使用DOI作为永久连结

引用含有DOI的文献

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。 如想对DOI的使用与概念有进一步了解，请参考 （ ） 。

DOI可强化引用精确性、增强学术圈连结，并给予使用者跨平台的良好使用经验，目前在全世界已有超过五千万个对象申请DOI。如想对DOI的使用与概念有进一步了解，请参考（）。