不同類型清潔劑之雞胚胎細胞體外毒性試驗與家兔眼角膜體內試驗之比較研究

A Comparison between the Cytotoxicity Test with Chicken Embryo Cells in Vitro and the Corneal Test with Rabbit Eyes in Vivo of Different Detergents

10.6946/ASRNL.198609.0021

趙磐華(Edward Chao)

清潔劑 ； Draize test ； Cornea Test ； Chicken Embryo cell ； Detergents

藥物食品檢驗局調查研究年報

4號（1986 / 09 / 01）

21 - 27

繁體中文

An anionic surfactant, sodium dodecylbenzene sulfonate (SDS), was administered directly to the corneal surface of albino rabbits, causing various irritations and histopathological changes to eyes and corneal membranes of the rabbits. The toxicity of four different detergents determined by this corneal test followed the order of cetyltrimethylammonium bromide (cationic)＞sodium deoxycholate (anionic)＞SDS (anionic)＞triton x-100(nonionic). These substances were also tested for cell toxicity in vitro using a primary culture of chicken embryo cells and the minimum cytotoxicity concentrations were found to be of the same level as in the corneal test, yet the doses required were much smaller. This method offers a very good means for screening the potentially irritating detergents without using live animals and can be used to select product formulations for studies in vivo.

An anionic surfactant, sodium dodecylbenzene sulfonate (SDS), was administered directly to the corneal surface of albino rabbits, causing various irritations and histopathological changes to eyes and corneal membranes of the rabbits. The toxicity of four different detergents determined by this corneal test followed the order of cetyltrimethylammonium bromide (cationic)＞sodium deoxycholate (anionic)＞SDS (anionic)＞triton x-100(nonionic). These substances were also tested for cell toxicity in vitro using a primary culture of chicken embryo cells and the minimum cytotoxicity concentrations were found to be of the same level as in the corneal test, yet the doses required were much smaller. This method offers a very good means for screening the potentially irritating detergents without using live animals and can be used to select product formulations for studies in vivo.