Liposomes-Coated Hydroxyapatite and Tricalcium Phosphate Implanted in the Mandibular Bony Defect of Miniature Swine

以微脂粒覆蓋氫氧磷灰石與磷酸三鈣植入迷你豬下顎骨缺損之研究

10.6452/KJMS.199704.0213

黃景勝(Jiing-Sheng Huang)；劉坤銘(Kun-Ming Liu)；陳慶長(Ching-Charng Chen)；何坤炎(Kun-Yen Ho)；吳逸民(Yi-Min Wu)；王俊欽(Chun-Chin Wang)；鄭裕民(Yuh-Ming Cheng)；柯文龍(Wen-Lung Ko)；劉振聲(Chen-Sheng Liu)；何雅萍(Ya-Pyng Ho)；王英斌(Yin-Pin

liosome ； hydroxyapatite ； tricalcium phosphate

The Kaohsiung Journal of Medical Sciences

13卷4期（1997 / 04 / 01）

213 - 228

英文

牙周疾病之症狀包括：牙齦炎、附著結締組織及齒槽骨的喪失。近年來，牙周疾病之治療隨著新的技術與新材料的開發已有相當的進展，然而，對於牙周病造成的骨缺損，在骨組織以及體積恢復，卻仍無十分可靠的修復材料與技術。微脂粒已被證實對類骨質細胞有促進生長用。本實驗將微脂粒覆蓋氫氧磷灰石與磷酸三鈣為植體，植入迷你豬蓋氫氧磷灰石與磷酸三鈣在迷你豬組織內具有良好的生物相容性。在第三週時植體均被緻密的結締織包圍，而第六週時植體旁已經可以見到新的骨質生成；然而跟純粹的氫氧磷灰石與磷酸三鈣植體比較，骨組織的鈣化程度較不完全，而且骨質生長亦較不足。此外將微脂粒混入洋菜膠中植入骨缺損的結果顯示：微脂粒在骨組織中具有良好的生物相容性，在第三週亦被結締組織包圍，在第六週則有骨痂形成，顯示微脂粒確實具有促進骨組織生長的能力。

Hydroxyapatite and tricalcium phosphate have been used as bone implants for some period of time. Now unfortunately, these materials have failed to become the nucleation sites for bone regeneration. We hypothesized that coating hydroxyapatite and tricalcium phosphate with negatively charged liposomes may improve the nucleation process for new bone formation. The present study was designed to test this hypothesis. Experiments were carried out in 15 minature swines’ mandibular angle with artificial bony defects. In each of the swine, the bony defects on one side were implanted with either liposomes coated with hydroxyapatite or liposomes coated with tricalcium phosphate, while the other side served as control. At the end of the third and sixth weeks following the operation, we observed result, took histology and radiographs of the operated area. The results showed that liposomes-coated materials were biocompatible and their clinical endpoint was enhanced. At the end of the third week, the implant material was surrounded by dense connective tissues. At the end of the sixth week, there were new bone formations near the implanted material. In addition, liposomes which were immobilized in agarose gel and implanted in the defects showed new bony bridge formation. These observations suggest that liposomes have the ability in promoting repair of osseous deficiencies.