题名

Effect of Gossypol on Intracellular Ca2+ Regulation in Human Hepatoma Cells

作者

Jin-Shiung Cheng;Yuk-Keung Lo;Jeng-Hsien Yeh;He-Hsiung Cheng;Chun-Peng Liu;Wei-Chuan Chen;Chung-Ren Jan

关键词

Ca2+ signaling ; fura-2 ; gossypol ; HA22/VGH cells ; hepatocytes

期刊名称

The Chinese Journal of Physiology

卷期/出版年月

46卷3期(2003 / 09 / 01)

页次

117 - 122

内容语文

英文

英文摘要

Gossypol is a natural toxicant present in cottonseeds, and is hepatotoxic to animals and human. The effect of gossypol on cytosolic free Ca2+ levels ([Ca2+]i) in HA22/VGH human hepatocytes was explored using fura-2 as a fluorescent Ca2+ indicator. Gossypol increased [Ca2+]i in a concentrationdependent manner with an EC50 value of 2 M. The Ca2+ signal was reduced by removing extracellular Ca2+ or by 10 M La3+, but was not affected by nifedipine, verapamil or diltiazem. Pretreatment with 1 M thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor) to deplete the endoplasmic reticulum Ca2+ partly reduced 10 M gossypol-induced Ca2+ release; and conversely pretreatment with gossypol abolished thapsigargin-induced Ca2+ release. The Ca2+ release induced by 10 M gossypol was not changed by inhibiting phospholipase C with 2 M U73122 or by depleting ryanodine-sensitive Ca2+ stores with 50 M ryanodine. Together, the results suggest that in human hepatocytes, gossypol induced a [Ca2+]i increase by causing store Ca2+ release from the endoplasmic reticulum in a phospholipase Cindependent manner, and by inducing Ca2+ influx.

主题分类 醫藥衛生 > 基礎醫學
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被引用次数
  1. Shieh, Pochuen,Liang, Wei-Zhe,Kuo, Daih-Huang,Kuo, Chun-Chi,Jan, Chung-Ren,Ho, Chin-Man,Chou, Chiang-Ting,Chien, Jau-Min(2014).The Mechanism of NPC-14686-Induced [Ca2+]i Rises and Non-Ca2+-Triggered Cell Death in MG63 Human Osteosarcoma Cells.中國生理學雜誌,57(3),158-166.
  2. Shieh, Pochuen,Lu, Ti,Liang, Wei-Zhe,Kuo, Daih-Huang,Jan, Chung-Ren,Ho, Chin-Man,Chou, Chiang-Ting,Chang, Hong-Tai(2014).Effects of Thymol on Ca2+ Homeostasis and Apoptosis in MDCK Renal Tubular Cells.中國生理學雜誌,57(2),90-98.
  3. Tsai, Jeng-Yu,Lu, Yi-Chau,Lu, Ti,Liu, Shiuh-Inn,Liou, Wen-Chin,Lin, Ko-Long,Liao, Wei-Chuan,Liang, Wei-Zhe,Jan, Chung-Ren,Hsu, Shu-Shong,Chou, Chiang-Ting,Chen, Wei-Chuan(2014).Rise of [Ca2+]i and Apoptosis Induced by M-3M3FBS in SCM1 Human Gastric Cancer Cells.中國生理學雜誌,57(1),31-40.
  4. Tseng, Hui-Wen,Tsai, Yi-Jen,Tsai, Peng-Chih,Shieh, Pochuen,Lin, Jia-Rong,Liang, Wei-Zhe,Kuo, Daih-Huang,Kuo, Chun-Chi,Jan, Chung-Ren,Hung, Ming-Chi,Horng, Chi-Ting,Ho, Chin-Man,Chou, Chiang-Ting,Cheng, Jin-Shiung,Chen, I-Li,Chang, Po-Min,Chang, Hong-Tai(2015).Effect of Methoxychlor on Ca2+ Homeostasis and Apoptosis in HA59T Human Hepatoma Cells.中國生理學雜誌,58(1),1-8.
  5. Wu, Ru-Fang,Tseng, Li-Ling,Shieh, Pochuen,Lin, Ko-Long,Liang, Wei-Zhe,Kuo, Daih-Huang,Kuo, Chun-Chi,Jan, Chung-Ren,Chou, Chiang-Ting,Cheng, Jin-Shiung,Chen, I-Li,Chang, Hong-Tai(2014).Effect of Fluoxetine on [Ca2+]i and Cell Viability in OC2 Human Oral Cancer Cells.中國生理學雜誌,57(5),256-264.
  6. Yu, Chia-Cheng,Shieh, Pochuen,Lu, Ti,Liang, Wei-Zhe,Kuo, Daih-Huang,Kuo, Chun-Chi,Jan, Chung-Ren,Ho, Chin-Man,Chou, Chiang-Ting,Chen, Wei-Chuan,Chang, Hong-Tai(2015).Effect of Antidepressant Doxepin on Ca2+ Homeostasis and Viability in PC3 Human Prostate Cancer Cells.中國生理學雜誌,58(3),178-187.