Participation of Caudal Ventrolateral Medulla in the Regulation of Gallbladder Motility in Rabbits

Feng-Yu Liu；Chuan-Yong Liu；Jing-Zhang Liu；Dong-Ping Xie

CVLM ； gallbladder pressure ； NMDA ； AMPA ； sympathetic nerve

The Chinese Journal of Physiology

47卷1期（2004 / 03 / 01）

1 - 6

英文

To investigate whether the caudal ventrolateral medulla (CVLM) participates in the regulation of gallbladder motility, we studied the effects of microinjection of L-glutamate and other agents into the CVLM on gallbladder pressure (GP) in anesthetized rabbits. A frog bladder connected with a force transducer was inserted into the gallbladder to record the change of GP. Microinjection of L-glutamate into the CVLM decreased GP, While micnoinjection of γ-amino-butyric acid (GABA) increased GP. Microinjection of ketamine, a noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist, into CVLM increased GP, while microinjection of 6-cyano-7-nitroquinoxaline-2, 3-(1H, 4H)-dione (CNQX), a competitive (±)-a-amino-3-hydroxy-5- methylisoxazole-4-propionic acid (AMPA)/kainate receptor antagonist, had no significant effect on GP. The effects of L-glutamate was abolished by ketainine, but not by CNQX. Intravenous injection of phentolamine or transection of the spinal cord eliminated the effects of L-glutamate on GP. These results indicate that [1] CVLM participated in the regulation of gallbladder motility; [2] endogenous L-glutamate in CVLM is involved in the regulation mediated by NMDA receptors, the output of which is sent through sympathetic nerve and x-adrenergic receptors.

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