Evaluation of Mouse Blastocyst Implantation Rate by Morphology Grading

Tzu-Chun Cheng；Chun-Chia Huang；Lii-Sheng Huang；Chung-I Chen；Maw-Sheng Lee；Jer-Yuh Liu

blastocyst ； implantation rate ； inner cell mass (ICM) ； trophectoderm (TE)

The Chinese Journal of Physiology

47卷1期（2004 / 03 / 01）

43 - 47

英文

The aim of our study is to observe the relationship between the blastocyst morphology and the implantation rate for mice. Mouse embryos obtained from the superovulated-ICR mice were cultured in vitro from 1-cell zygotes to blastocysts. Mouse blastocysts were then classified into 3 grades: grade Ⅰ, small blastocysts; grade Ⅱ, large blastocysts; grade Ⅲ, hatching blastocysts. They were independently transferred into the uterus of recipient females mated with vasectomized male mice on 96 hours after the zygotes were cultured in vitro. The successful implantation was checked by injection of Chicago Sky Blue 6B on the second day after embryo transfer. Although there was no significant difference in the implantation rates between the grade Ⅲ and grade Ⅱ, grade Ⅰ was significantly decreased, as compared with grade Ⅲ. Grade Ⅰ and grade Ⅱ was also significantly decreased in both the diameter of bias tocysts and cell number of inner cell mass (1CM) and trophectoderm (TE), as compared with grade Ⅲ. These findings indicate that the expanded and hatching blastocyst selections for embryo transfer in in vitro fertilization were evaluated with the high implantation rate.

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