题名

IgA Immune Complex Blunts the Contraction of Cultured Mesangial Cells through the Inhibition of Protein Kinase C and Intracellular Calcium

作者

Jong-Shiaw Jin；Chen-Wen Yao；Shuk-Man Ka；Ting-Yu Chin；Sheau-Huei Chueh；Herng-Sheng Lee；Lai-Fa Sheu；Yeh-Feng Lin；Wei-Hwa Lee；Ann Chen

关键词

mesangial cells ； contractility ； protein kinase C ； intracellular calcium

期刊名称

The Chinese Journal of Physiology

卷期/出版年月

47卷2期（2004 / 06 / 01）

页次

79 - 87

内容语文

英文
英文摘要

The effects of IgA immune complex (IgA-IC) on the contractile function of cultured mesangial cells were measured by the changes in planar surface area in response to treatment with agonists. Incubation of mesangial cells with IgA-IC for 24 hours significantly decreased the contractile responses to angiotensin Ⅱ(10^(-6) M) and phorbol 12-myristate 13-acetate (PMA, 10^(-6) M). Pretreatment of mesangial cells with the protein kinase C (PKC) inhibitor, chelerythrine (10^(-6) M), eliminated the difference in contractile responses to angiotensin II or PMA between the control and IgA-IC groups indicating IgA-IC may inhibit the activity of PKC. The contractile responses to ionomycin were not significantly different between IgA-IC treated and control mesangial cells, suggesting that the contractile machinery is not impaired by IgA-IC. Intracellular calcium, [Ca(supscript +2)](subscript i) measured by changes in fura-2 level in response to ATP or bradykinin, was significantly inhibited in IgA-IC treated mesangial cells, compared to control cells. In contrast, treatment with thapsigargin did not result in significant differences in [Ca(supscript +2)] (subscript i) between IgA-IC and control mesangial cells, suggesting that a negligible role of endoplasmic reticulum in the effects of IgA-IC. Using PKC specific antibodies, IgA-IC significantly increased the particulate fraction of PKC-l of mesangial cells to 141±13% of control, without significantly changing the protein content of PKC-α, -δ and –λ in the cytosolic and particulate fractions. In summary, IgA-IC inhibits the contractile responses of cultured mesangial cells to agonists by inhibiting the activation of PKC and [Ca(superscript 2+)](subscript i).
主题分类 醫藥衛生 > 基礎醫學
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