Uric Acid and Urea in Human Sweat

Chien-Tsai Huang；Mei-Lien Chen；Li-Ling Huang；I-Fang Mao

sweat ； uric acid ； urea ； uric acid excretion

The Chinese Journal of Physiology

45卷3期（2002 / 09 / 30）

109 - 115

英文

The present study investigated whether thermal sweating may relieve elevated concentrations of serum uric acid or urea. Concentrations of uric acid and urea were measured in the sweat of sixteen male volunteers, who were treated with external heat after one hour of intense physical exercise. The same analytes were also measured in their urine and serum samples. Furthermore, creatinine and some electrolytes were determined in these specimens. The results show that the concentration of uric acid in the sweat is 24.5μmol/L, which is only 6.3% of that in serum. The concentration of urea in the sweat is 22.2mmol/L, which is 3.6 times that in serum. The results indicate that sweat uric acid concentration is quite minimal, and the estimated total uric acid excretion per day in normal physiological range is insignificant. However, the level of sweat urea was found at a much higher concentration than the serum level. No correlation could he established between the level of uric acid in sweat and in serum. There was also no correlation between the level of urea in sweat and that in serum. These results suggest it would not be effective to relieve the elevated serum uric acid concentration by thermal sweating when the renal excretion of uric acid is partly compromised. Nevertheless, the potential of urea excretion via profuse sweating is apparent particularly when the kidneys are damaged or their function is impaired. These findings also suggest that persons who take vigorous exercise or are exposed to hot environments should be well advised to drink adequate fluids since heavy sweating excretes only minimal uric acid, accompanied by significant diminution of urinary output and diminished urinary excretions of uric acid, which may induce elevated levels of serum uric acid.

1. Al-Tamer, Y.Y.,Hadi, E.A.,Al-Badrani, I.I.(1997).Sweat urea, uric acid and creatinine concentrations in uraemic patients.Urol. Res,25,337-340.
2. Boysen, T.C.,Yanagawa, S.,Sato, F.,Sato, K. A.(1984).modified anaerobic method of sweat collection.J. Appl. Physiol,56,1302-1307.
3. Butler, A.R.(1975).The Jaffe reaction: identification of the coloured species.Clin. Chim, Acta.,59,227-232.
4. Diamond, H.S.,Lazarus, R.,Kaplan, E.(1972).Effect of urine flow rate on uric acid excretion in man.Arthritis Rheum,15,338.
5. Emrich, H.M.,Stoll, E.,Friolet, B.,Colombo, J.P.,Richrerich, R.,Rossi, E.(1968).Sweat composition in relation to rate of sweating in patients with cystic fibrosis of the pancreas.Pedictr Rex,2,464-478.
6. Engle, J.E.,Steele, T.H.(1976).Variation urate excretion with urine flow in normal man.Nephrom,16,50-56.
7. Fellmann, N.,Labbe, A.,Gachon, A.M.,Coudert, J.(1985).Thermal sweat lactate in cystic Fibrosis and in normal children.Eur. J. Appl. Physiol,54,511-516.
8. Fossati, P.,Prencipe, L.,Berti, G.(1980).Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.Clin. Chem,26,227-231.
9. Garden, J.W.(1966).Plasma and sweat histamine concentrations after heat exposure arid physical exercise.J. Appl. Physiol,21,631-635.
10. Grahame, R.,Scott, J.T.(1970).Clinical survey of 354 patients with gout.Ann. Rheum. Dis.,29,461-468.
11. Guyton, A.C.,Hall, J. E.(1997).Human Physiology and Mechanisms of Disease.Philadelphia, PA:W.B. Saunders.
12. Kleinbaum, D.G.,Kupper, L.L.,Muller, K.E.(1986).Applied Regression Analysis and Other Multivariable Method.Boston, MA:PWSKENT.
13. Kumives, G.K.,Robinson, S.,Roberts, J.T.(1966).Urea transfer across the sweat gland.J. Appl. Physiol.,21,1681-1684.
14. Maesaka, J.K.,Fishbne, S.(1998).Regulation of renal urate excretion: a critical review.Am. J. kidney. Dis.,32,917-933.
15. Mangos, J.A.(1973).Transductal fluxes of Na, K and water in the human eccrine sweat glands.Am. J. Physiol,224,1235-1240.
16. Pilardeau, P.A.,Chalumeau, M.T.,Harichax, P.(1988).Eeffect of physical training on exercise-induced sweating in man.J. Sport. Med.,28,247-252.
17. Sampson, E.J.,Baird, M.A.,Burtis, C.A.,Smith, E.M.,Wine, D.L.,Bayse, D.D.(1980).A coupled-enzyme equilibrium method for measuring urea in serum: optimization and evaluation of the AACC study group on urea candidate reference method.Clin, Chem,26,816-826.
18. Sato, K.(1973).Sweat induction from an isolated ecerine sweat gland.Am. J. Physiol,225,1147-1151.
19. Sato, K.(1977).The physiology, pharmacology, and biochemistry of the eccrine sweat gland.Rev, Physiol, Biochem, Pharmacol,79,51-131.
20. Sato, K.,Dobson, R.L.(1970).The effect of intracutaneous D-aldoster-one and hydrocortisone on the human eccrine sweat gland function.J. Invest, Dermutol,54,450-459.
21. Sato, K.,Kang, W.H.,Saga, K.,Sato, K.T.(1989).Biology of sweat glands and their disorders, L normal sweal gland function.J. Am. Acad. Dermatol,20,537-563.
22. Taylor, R.P.,Polliack, A.A.,Bader, D.L.(1994).The analysis of metabolites in human sweat: analytical methods and potenti al application to investigation of pressure ischaemia of soft tissues.Ann, Clin, Biochem,31,18-24.
23. Tietz, N.W.,Pruden, E.L.,Siggaard-Andrsen, O.,Burtis, C.A.,Ashwood, E.R.(edited)(1994).Tierz Textbook of Clinical Chemistry.Philadelphia, PA:W.B. Saunders.
24. Tortoba, G.J.(1983).Principles of Human Anatomy.New York, NY:Harper & Row.
25. Webster, H.L.,Barlow, W.K.(1981).New approach to cystic fibrosis diagnosis by use of an improved sweat induction collection system and osmometry.Clin, Chem,27,385-387.

1. Mao, I-fang,Huang, Li-ling,Huang, Chien-tsai,Chen, Mei-lien(2010).Effects of Profuse Sweating Induced by Exercise on Urinary Uric Acid Excretion in a Hot Environment.中國生理學雜誌,53(4),254-261.