Mast Cell Stabilizator and Antioxidant Effects of Epidermal Growth Factor (EGF) on Gastric Mucosal Injury Induced by Ethanol in Rats

Serdar Erkasap；Nilufer Erkasap；Erinc Aral；Tulay Koken；Ahmet Kahraman；Yasemin Aydin；Sezgin Yilmaz；Ersin Ates

epidermal growth factor (EGF) ； ethanol induced gastric mucosal injury ； mast cell ； oxidative stress

The Chinese Journal of Physiology

48卷1期（2005 / 03 / 01）

1 - 6

英文

The role of epidermal growth factor (EGF), a polypeptide containing 53 amino acids, on protection and repair of ethanol-induced gastric mucosal injury was investigated in rats. In addition, the effects of EGF on the gastric damage were evaluated histopathologically. We used 48 Spraque-Dawley rats which were divided three groups as control rats, ethanol treated rats and ethanol + EGF treated rats. The ethanol group was given a gastric gavage containing 1 ml of 80% ethanol (v/v) prepared in distilled water. EGF (100μg/kg) was given by intragastric gavage 30 min before the administration of ethanol. We studied histopathological evaluation and the histochemical heterogeneity of mast cells and its degree of degranulation. Besides, gastric tissue malondialdehyde (MDA), protein sulfhydry1 groups (SH), and protein carbonyl levels were measured. EGF treatment stabilized mast cells degranulation and had lower polymorphonuclear leukocytes (PMNL) infiltration, ulcer index, histamine, and MDA; protein carbonyl levels were also lower, compared to the non-treated animals. EGF exerts a protective effect on gastric mucosa to ethanol-induced gastric injury probably through antioxidant and mast cell stabilizing mechanism.

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