Overexpression of Anion Exchanger 2 in Human Hepatocellular Carcinoma

Trang-Tiau Wu；Yi-Hsien Hsieh；Cheng-Chung Wu；Jen-Hsiang Tsai；Yih-Shou Hsieh；Chih-Yang Huang；Jer-Yuh Liu

DDRT-PCR ； hepatocellular carcinoma ； anion exchanger 2

The Chinese Journal of Physiology

49卷4期（2006 / 08 / 31）

192 - 198

英文

To compare gene expression patterns between the poorly-differentiated (HA22T/VGH) and well-differentiated (HepG2) hepatocellular carcinoma cells, messenger RNA was isolated form both kinds of cells and subjected to differential displays reversing transcriptase-polymerase chain reaction (DDRTPCR) technology. Gene fragments showing difference in the expression were recovered, reamplified, cloned and sequenced. Anion exchanger 2 (AE2), an isoform of band 3 protein, was identified and chosen for further characterization. AE2 was strongly expressed in HA22T/VGH cells, while it was little expressed in the well-differentiated hepatocellular carcinoma cells (PLC/PRF5 and HepG2) or little in the normal liver cell (Chang liver). In the 28 pairs of HCC and adjacent non-tumor tissues, levels in the cancer tissue (32.7±5.0) were significantly higher than that in the adjacent non-tumor tissue (9.1±2.4) (P＜0.01). Moreover, 19 cases (68%) showed over-expression of AE2 in HCC tissues, 3 cases were similar in both tissues, and 6 cases exhibited little or undetectable signals. Twenty cases (71%) of adjacent normal tissue showed little or undetectable signals. The results indicated that overexpression of AE2 may be involved in the development of human HCC.

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