Effects of Anti-Tumor Necrosis Factor-α and Anti-Intercellular Adhesion Molecule-1 Antibodies on Ischemia/Reperfusion Lung Injury

Chi-Huei Chiang

tumor necrosis factor-α TNF-α ； intercellular adhesion molecules ICAM-1 ； ischemia and reperfusion lung injury

The Chinese Journal of Physiology

49卷5期（2006 / 10 / 31）

266 - 274

英文

Inhibition of neutrophil activation and adherence to endothelium by antibodies to tumor necrosis factor-α (TNF-α) and intercellular adhesion molecules (ICAM-1), respectively, might attenuate ischemia-reperfusion injury (I/R). I/R was conducted in an isolated rat lung model. Anti-TNF-α antibody and/or anti-ICAM-1 antibody were added before ischemia or after reperfusion. Hemodynamic changes, lung weight gain (LWG), capillary filtration coefficients (Kfc), and pathologic changes were assessed to evaluate the severity of I/R. The LWG, Kfc, pathological changes and lung injury score of treatment groups with anti-TNF-α antibody treatment, either pre-ischemia or during reperfusion, were less than those observed in control groups. Similar findings were found in group treated with anti-ICAM-1 antibody or combination therapy during reperfusion. In contrast, pre-I/R treatment with anti-ICAM-1 antibody induced severe lung edema and failure to complete the experimental procedure. No additional therapeutic effect was found in combination therapy. We conclude that TNF-α and ICAM-1 play important roles in I/R. Anti-TNF-α antibody has therapeutic and preventive effects on I/R. However, combined therapy with anti-TNF-α antibody and anti-ICAM-1 antibody may have no additive effect and need further investigation.

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