Tissue Microarray-determined Expression Profiles of Cyclooxygenase-2 in Colorectal Adenocarcinoma: Association with Clinicopathological Parameters

Chi-Ying Wu；Wei-Hwa Lee；Jia-Yi Wang；Hung Chiang；Junn-Liang Chang；Wen-Chiuan Tsai；Lai-Fa Sheu；Jong-Shiaw Jin

adenocarcinoma ； colorectal ； cyclooxygenase-2 ； cytokeratin 20

The Chinese Journal of Physiology

49卷6期（2006 / 12 / 01）

298 - 304

英文

Accumulated evidence reveals that increased cyclooxygenase-2 (COX-2) is involved in the development of colorectal cancer. Our purpose was to quantitate COX-2 expression in colorectal cancers using tissue microarray analysis and look for an association with clinicopathological stage. Immunohistochemical analysis of COX-2 was performed in tissue microarray slides containing 90 specimens including 32 well-differentiated, 35 moderately differentiated, and 23 poorly differentiated colorectal adenocarcinomas. All colorectal adenocarcinomas showed significant immunohistochemical expression of COX-2 when compared to normal colon epithelia. However, there was no significant difference in immunostaining scores between poorly, moderately, and well-differentiated tumors (195±28, 214±26 and 200±24, respectively). The COX-2 immunostaining score correlated significantly with T stage (P＜0.05) but not with N or M stage. The positive expression rates of CK20 were 97% for welldifferentiated, 94% for moderately differentiated, and 65% for poorly differentiated colorectal adenocarcinomas, suggesting that CK20 may not be an effective discriminator between poorly differentiated colorectal adenocarcinoma and metastatic adenocarcinoma.

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