Oxidative and Nitrosative Mediators in Hepatic Injury Caused by Whole Body Hyperthermia in Rats

Jye-Fang Lee；David Wang；Yung-Hsiang Hsu；Hsing I Chen

whole body hyperthermia ； heatstress ； hepatic injury ； nitric oxide ； nitric oxide synthase ； free radical ； proinflammatory cytokines ； nitrotyrosine ； heat shock protein

The Chinese Journal of Physiology

51卷2期（2008 / 04 / 01）

85 - 93

英文

The involvement of oxidative and nitrosative mediators in liver injury caused by heat stress remains unclear. This study aim to elucidate the role of endothelial nitric oxide synthase (eNOS), and inducible NOS (iNOS)-derived NO and nitrotyrosine in the whole-body hyperthermia (WBH)-induced liver injury. Rats were anesthetized with intraperitoneal pentobarbital, and were exposed to a heating lamp for 60 min to raise the core temperature to 42.5℃. The rats were maintained at the hyperthermic state for an additional 50 min. Blood urea nitrogen, creatinine, aspartate aminotransferase, alanine aminotransferase, lactic dehydrogenase, creatine phosphokinase, amylase, lipase, nitrate/nitrite, methyl guanidine, and proinflammatory cytokines (tumor necrosis factor(subscript α), interleukin-1(subscript β) and interleukin-10) were measured before and 14h after hyperthermia. Immunohistochemical staining was employed to detect the eNOS, iNOS and nitrotyrosine levels. Western blotting was used to examine the expression of heatshock 70 (HSP 70). Histopathological examination of the liver tissue was performed. WBH caused liver injury accompanied with significant increases in biochemical factors, nitrate/nitrite, methyl guanidine, and proinflammatory cytokines. In addition, WBH enhanced the eNOS, iNOS, nitrotyrosine and HSP 70 levels. WBH caused hepatic injury. The pathogenetic mechanism is likely mediated through the NOS-derived NO, free radical, proinflammatory cytokines and nitrotyrosine. The enhanced expression of HSP 70 may play a protective role.

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