Survival and Differentiation of Neuroepithelial Stem Cells following Transplantation into the Lateral Ventricle of Rats

Jin-Hao Sun；Pei-Hui Zheng；Ling-Ling Wang；Jing-Jing Li；Jia-Zeng Wang；Qi Wu；Tao Song；Wen-Jing Liu；Ying-Mao Gao

embryonic ； neuroepithelial cells ； lateral ventricle ； transplantation

The Chinese Journal of Physiology

51卷4期（2008 / 08 / 01）

247 - 251

英文

Neuroepithelial stem cells (NEPs) demonstrate a high potential for self-renewal and differentiation during embryonic development. To explore the survival and differentiation of NEPs in vivo, we isolated NEPs from green fluorescence protein (GFP) transgenic embryos and transplanted into the lateral ventricle of rats. In vitro culture, NEPs proliferated into neurospheres and differentiated into both neurons and glia. When transplanted into the lateral ventricle of rats, these GFP positive NEPs (GFP+ NEPs) survived and attached to the wall of ventricle. Moreover, grafted cells differentiated into neuronspecific enolase (NSE) positive neurons and glial fibrillary acidic protein (GFAP) positive astrocytes and migrated into the host brain. Thus, our results indicate that NEPs can survive and differentiate into neurons and astrocytes in the lateral ventricle following transplantation.

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