Hyporeactivity of Renal Artery to Angiotensin Ⅱ

Huang-Ping Yu；Jee-Ching Hsu；Chia-Hung Yen；Yunn-Hwa Ma；Ying-Tung Lau

cecal ligation and puncture ； sepsis ； renal blood flow ； vascular response ； nitric oxide

The Chinese Journal of Physiology

51卷5期（2008 / 10 / 01）

301 - 307

英文

Although reduced vascular reactivity to vasoconstrictors has been well documented, it is not clear whether renal blood flow (RBF) and renal vascular response exhibit the same pattern following sepsis. We examined RBF and renal vascular response during early sepsis. Male Sprague-Dawley rats underwent cecal ligation and puncture (CLP)-induced sepsis. At 5 h after CLP or sham operation, RBF and plasma nitric oxide (NO) concentration were measured. Moreover, angiotensin Ⅱ (50 ng/kg body weight) was employed to evaluate the renal vascular response (n=12 rats/group). The results showed that CLP caused higher heart rate (HR), RBF and lower renal vascular resistance (RVR). In addition, plasma nitrite-nitrate (NOx) increased significantly after CLP. After angiotensin Ⅱ infusion, maximal response in mean blood pressure (MBP), RBF and RVR were less in CLP rats. Thus, we found that CLP induced hyporeactivity of renal artery together with overproduction of NO during an early stage of sepsis.

1. Boffa, J.,Arendshorst, W.J.(2005).Maintenance of renal vascular reactivity contributes to acute renal failure during endotoxemic shock.J. Am. Soc. Nephrol.,16,117-124.
2. Chauhan, S.D.,Seggara, G.,Vo, P.A.,Macallister, R.J.,Hobbs, A.J.,Ahluwalia, A.(2003).Protection against lipopolysaccharide-induced endothelial dysfunction in resistance and conduit vasculature of iNOS knockout mice.FASEB J.,17,773-775.
3. Colasanti, M.,Suzuki, H.(2000).The dual personality of NO.Trends Pharmacol. Sci.,21,249-252.
4. da Silva-Santos, J.E.,Terluk, M.R.,Assreuy, J.(2002).Differential involvement of guanylate cyclase and potassium channels in nitric oxide-induced hyporesponsiveness to phenylephrine in endotoxemic rats.Shock,17,70-76.
5. Dubinion, J.H.,Mi, Z.,Jackson, E.K.(2006).Role of renal sympathetic nerves in regulating renovascular responses to angiotensin II in spontaneously hypertensive rats.J. Pharmacol. Exp. Ther.,317,1330-1336.
6. Dubinion, J.H.,Mi, Z.,Zhu, C.,Gao, L.,Jackson, E.K.(2006).Pancreatic polypeptide-fold peptide receptors and angiotensin II-induced renal vasoconstriction.Hypertension,47,545-551.
7. Gardiner, S.M.,Kemp, P.A.,March, J.E.,Bennett, T.(1995).Cardiac and regional haemodynamics, inducible nitric oxide synthase (NOS) activity, and the effects of NOS inhibitors in conscious endotoxaemic rats.Brit. J. Pharmacol.,116,2005-2016.
8. Groeneveld, A.B.J.,Bronsveld, W.,Thijs, L.G.(1986).Hemodynamic determinants of mortality in human septic shock.Surgery,99,140-153.
9. Jackson, E.K.,Herzer, W.A.,Kost, C.K.,Vyas, S.J.(2001).Enhanced interaction between renovascular α2-adrenoceptors and angiotensin II receptors in genetic hypertension.Hypertension,38,353-360.
10. Joannidis, M.(2006).Acute kidney injury in septic shock-do not under-treat!.Intens. Care Med.,32,18-20.
11. Julou-Schaeffer, G.,Gray, G.A.,Fleming, I.,Schott, C.,Parrat, J.R.,Stoclet, J.C.(1991).Activation of the L-arginine-nitric oxide pathway is involved in vascular hyporeactivity induced by endotoxin.J. Cardiovasc. Pharmacol.,17,207-212.
12. Julou-Schaeffer, G.,Gray, G.A.,Fleming, I.,Schott, C.,Parrat, J.R.,Stoclet, J.C.(1990).Loss of vascular responsiveness induced by endotoxin involves L-arginine pathway.Am. J. Physiol.,259,1038-1043.
13. Klenzak, J.,Himmelfarb, J.(2005).Sepsis and the kidney.Crit. Care Clin.,21,211-222.
14. McKenna, T.M.(1992).Recovery of vascular tissue contractile function during sustained endotoxin exposure.Am. J. Physiol.,263,1628-1631.
15. Mikawa, K.,Nishina, K.,Kodama, S.,Obara, H.(2001).Propofol attenuates diaphragmatic dysfunction induced by septic peritonitis in hamsters.Anesthesiology,94,652-660.
16. Moncada, S.,Higgs, A.(1993).The L-arginine-nitric oxide pathway.N. Engl. J. Med.,329,2002-2012.
17. Palmer, R.M.J.(1993).The discovery of nitric oxide in the vessel wall. A unifying concept in the pathogenesis of sepsis.Arch. Surg.,128,396-401.
18. Pastor, C.M.,Billiar, T.R.(1995).Nitric oxide causes hyporeactivity to phenylephrine in isolated perfused livers from endotoxin-treated rats.Am. J. Physiol.,268,177-182.
19. Piepot, H.A.,Groeneveld, A.B.,van Lambalgen, A.A.,Sipkema, P.(2002).The role of inducible nitric oxide synthase in lipopolysaccharide-mediated hyporeactivity to vasoconstrictors differs among isolated rat arteries.Clin. Sci. (Lond),102,297-305.
20. Schneider, F.,Bucher, B.,Schott, C.,Andre, A.,Julou-Schaeffer, G.,Stoclet, J.C.(1994).Effect of bacterial lipopolysaccharide on function of rat small femoral arteries.Am. J. Physiol.,266,191-198.
21. Schneider, F.,Schott, C.,Stoclet, J.C.,Julou-Schaeffer, G.(1992).Larginine induces relaxation of small mesenteric arteries from endot-oxin-treated rats.Eur. J. Pharmacol.,211,269-272.
22. Schrier, R.W.,Wang, W.(2004).Acute renal failure and sepsis.N. Engl. J. Med.,351,159-169.
23. Sibbald, W.J.,Fox, G.,Martin, C.(1991).Abnormalities of vascular reactivity in the sepsis syndrome.Chest,100,155-159.
24. Wakabayashi, I.,Hatake, K.,Kakishita, E.,Nagai, K.(1987).Diminution of contractile response of the aorta from endotoxin-injected rats.Eur. J. Pharmacol.,141,117-122.
25. Wallis, G.,Brackett, D.,Lerner, M.,Kotake, Y.,Bolli, R.,McCay, P.B.(1996).In vivo spin trapping of nitric oxide generated in the small intestine, liver, and kidney during the development of endotoxemia: A time-course study.Shock,6,274-278.
26. Wang, P.,Chaudry, I.H.(1996).Mechanism of hepatocellular dysfunction during hyperdynamic sepsis.Am. J. Physiol.,270,927-938.
27. Wichterman, K.A.,Baue, A.E.,Chaudry, I.H.(1980).Sepsis and septic shock-A review of laboratory models and a proposal.J. Surg. Res.,29,189-201.
28. Wu, C.C.,Yen, M.H.(1999).Higher level of plasma nitric oxide in spontaneously hypertensive rats.Am. J. Hypertens.,12,476-482.
29. Yang, S.,Chung, C.S.,Ayala, A.,Chaudry, I.H.,Wang, P.(2002).Differential alterations in cardiovascular responses during the progression of polymicrobial sepsis in the mouse.Shock,17,55-60.
30. Yang, S.,Cioffi, W.G.,Bland, K.I.,Chaudry, I.H.,Wang, P.(1999).Differential alterations in systemic and regional oxygen delivery and consumption during the early and late stages of sepsis.J. Trauma,47,706-712.
31. Yu, H.P.,Lui, P.W.,Hwang, T.L.,Yen, C.H.,Lau, Y.T.(2006).Propofol improves endothelial dysfunction and attenuates vascular superoxide production in septic rats.Crit. Care Med.,34,453-460.