Supplementary Catechins Attenuate Cooking-Oil-Fumes-Induced Oxidative Stress in Rat Lung

10.4077/CJP.2009.AMH022

Chao-Huei Yang；Chun-Yao Lin；Joan-Hwa Yang；Shaw-Yih Liou；Ping-Chia Li；Chiang-Ting Chien

catechins ； reactive oxygen species ； HSP70 ； cooking-oil-fumes ； apoptosis ； lung

The Chinese Journal of Physiology

52卷3期（2009 / 06 / 01）

151 - 159

英文

Cooking-oil-fumes containing toxic components may induce reactive oxygen species (ROS) to oxidize macromolecules and lead to acute lung injury. Our previous study showed that a decaffineated green tea extract containing (+)-catechin, (-)-epicatechin, (+)-gallocatechin, (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate can inhibit oxidation, inflammation, and apoptosis. We determined whether the catechins supplement may reduce cooking-oil-fumes-induced acute lung injury in rat. In the urethane-anesthetized Wistar rat subjected to 30-120 min of cooking-oil-fumes exposure, blood ROS significantly increased in the recovery stage. After 30-min cooking-oil-fumes exposure, the enhanced blood ROS level further increased in a time-dependent manner during the recovery stage (321±69 counts/10 s after 1 h, 540±89 counts/10 s after 2 h, and 873±112 counts/10 s after 4 h). Four hours after 30-min cooking-oil-fumes exposure, lung lavage neutrophils and ROS as well as lung tissue dityrosine and 4-hydroxy-2-nonenal increased significantly. Two weeks of catechins supplememnt significantly reduced the enhanced lavage ROS, lung dityrosine and 4-hydroxy-2-nonenal level. Cooking-oil-fumes-induced oxidative stress decreased lung Bcl-2/Bax ratio and HSP70 expression, but catechins treatment preserved the downregulation of Bcl-2/Bax ratio and HSP70 expression. We conclude that catechins supplement attenuates cooking-oil-fumes-induced acute lung injury via the preservation of oil-smoke induced downregulation of antioxidant, antiapoptosis, and chaperone protein expression.

1. Chang, L.W.,Lo, W.S.,Lin, P.(2005).Trans, trans-2,4-decadienal, a product found in cooking-oil-fumes, induces cell proliferation and cytokine production due to reactive oxygen species in human bronchial epithelial cells.Toxicol. Sci.,87,337-343.
2. Chen, W.C.,Hayakawa, S.,Shimizu, K.,Chien, C.T.,Lai, M.K.(2004).Catechins prevents substance P-induced hyperactive bladder in rats via the downregulation of ICAM and ROS.Neurosci. Lett.,367,213-217.
3. Cherng, S.H.,Huang, K.H.,Yang, S.C.,Wu, T.C.,Yang, J.L.,Lee, H.(2002).Human 8-oxoguanine DNA glycosylase 1 mRNA expression as an oxidative stress exposure biomarker of cooking-oilfumes.J. Tox. Env. H. Part A.,65,265-278.
4. Chien, C.T.,Chang, T.C.,Tsai, C.Y.,Shyue, S.K.,Lai, M.K.(2005).Adenovirus-mediated bcl-2 gene transfer inhibits renal ischemia/reperfusion induced tubular oxidative stress and apoptosis.Am. J. Transplant.,5,1194-1203.
5. Chien, C.T.,Lee, P.H.,Chen, C.F.,Ma, M.C.,Lai, M.K.,Hsu, S.M.(2001).De novo demonstration and co-localization of free-radical production and apoptosis formation in rat kidney subjected to ischemia/reperfusion.J. Am. Soc. Nephrol.,12,973-982.
6. Chien, C.T.,Shyue, S.K.,Lai, M.K.(2007).Bcl-xL augmentation potentially reduces ischemia/reperfusion induced proximal and distal tubular apoptosis and autophagy.Transplantation,84,1183-1190.
7. Choi, Y.T.,Jung, C.H.,Lee, S.R.,Bae, J.H.,Baek, W.K.,Suh, M.H.(2001).The green tea polyphenol (-)-epigallocatechin gallate attenuates β-amyloid-induced neurotoxicity in cultured hippocampal neurons.Life Sci.,70,603-614.
8. Coggon, D.,Panneft, B.,Osmond, C.,Acheson, E.D.(1986).A survey of cancer and occupation in young and middle aged men. I. Cancers of the respiratory tract.Brit. J. Int. Med.,43,332-338.
9. Dung, C.H.,Wu, S.C.,Yen, G.C.(2006).Genotoxicity and oxidative stress of the mutagenic compounds formed in fumes of heated soybean oil, sunflower oil and lard.Toxicol. In Vitro.,20,439-447.
10. El-Farra, M.A.,Saka, S.,Guellati, M.,Bouzerna, N.(2000).The role of glutathione in the detoxication of lipid peroxides arising from heated oils.Acad. Bulg. Sci.,53,91-94.
11. Fan, H.,Sun, B.,Gu, Q.,Lafond-Walker, A.,Cao, S.,Becker, L.C.(2002).Oxygen radicals trigger activation of NF-κB and AP-1 and upregulation of ICAM-1 in reperfused canine heart.Am. J. Physiol. Heart Circ. Physiol.,282,1778-1786.
12. Gossart, S.,Cambon, C.,Orfila, C.,Seguelas, M.H.,Lepert, J.C.,Rami, J.(1996).Reactive oxygen intermediates as regulators of TNF-α production in rat lung inflammation induced by silica.J. Immunol.,156,1540-1548.
13. Gromadzinska, J.,Skolowska, M.,Wasowicz, W.(1998).Glutathione peroxidase activity, lipid peroxides and selenium concentration in various rat organs.Biomed. Biochim. Acta.,47,19-24.
14. Hazen, S.L.,Heinecke, J.W.(1997).3-Chlorotyrosine, a specific marker of MPO-catalyzed oxidation, is markedly elevated in LDL isolated from human atherosclerotic intima.J. Clin. Invest.,99,2075-2081.
15. Henning, S.M.,Fajardo-Lira, C.,Lee, H.W.,Youssefian, A.A.,Go, V.L.,Heber, D.(2003).Catechin content of 18 teas and a green tea extract supplement correlates with the antioxidant capacity.Nutr. Cancer.,5,226-235.
16. Hertog, M.G.,Feskens, E.J.,Hollman, P.C.,Katan, M.B.,Kromhout, D.(1993).Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.Lancet,342,1007-1011.
17. Ho, C.T.,Carlin, J.,Huang, T.C.,Hwang, L.S.,Hau, L.B.,Martens, M. (edited),Dalen, G.A. (edited),Russwurm, H. (edited)(1987).Flavor Science and Technology.Wiley, PA:Chichester.
18. Huang, K.C.,Yang, C.C.,Lee, K.T.,Chien, C.T.(2003).Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water.Kidney Int.,64,704-714.
19. Hung, H.S.,Wu, W.J.,Cheng, Y.W.,Wu, T.C.,Chang, K.L.,Lee, H.(2007).Association of cooking-oil-fumes exposure with lung cancer: involvement of inhibitor of apoptosis proteins in cell survival and proliferation in vitro.Mutat. Res.,628,107-116.
20. Katiyar, S.K.,Mukhtar, H.(1997).Tea antioxidants in cancer chemoprevention.J. Cell Biochem.,27,59-67.
21. Ko, Y.C.,Cheng, L.S.,Lee, C.H.,Huang, J.J.,Huang, M.S.,Kao, E.L.(2000).Chinese food cooking and lung cancer in women nonsmokers.Am. J. Epidemiol.,151,140-147.
22. Kode, A.,Yang, S.R.,Rahman, I.(2006).Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells.Respir. Res.,7,1-20.
23. Lam, W.K.(2005).Lung cancer in Asian women-the environment and genes.Respirology,10,408-417.
24. Lund, E.,Borgan, J.(1987).Increased lung cancer mortality among Norwegign cooks.Scand. J. Work Environ. Health,13,156-161.
25. Miyazawa, T.(2000).Absorption, metabolism and antioxidative effects of tea catechin in humans.Biofactors,13,55-59.
26. Okabe, S.,Ochiai, Y.,Aida, M.,Park, K.,Kim, S.J.,Nomura, T.(1999).Mechanistic aspects of green tea as cancer preventive on human stomach cancer cell lines.Jpn. J. Cancer Res.,90,733-739.
27. Pu, Y.,Yin, L.,Yuan, H.,Liang, G.(2002).Oxidative stress of cooking-oil-fume on rat type II lung cells.Wei Sheng Yan Jiu.,31,85-87.
28. Salahudeen, A.K.,Huang, H.,Joshi, M.,Moore, N.A.,Jenkins, J.K.(2003).Involvement of the mitochondrial pathway in cold storage and rewarming-associated apoptosis of human renal proximal tubular cells.Am. J. Transplant.,3,273-281.
29. Winterbourn, C.C.,Pichorner, H.,Kettle, A.J.(1997).Myeloperoxidase-dependent generation of a tyrosine peroxide by neutrophils.Arch. Biochem. Biophys.,338,15-21.
30. Wu, S.C.,Yen, G.C.(2004).Effects of cooking-oil-fumes on the genotoxicity and oxidative stress in human lung carcinoma (A-549) cells.Toxicol. In Vitro.,18,571-580.
31. Yagi, K.(1984).Assay for blood plasma or serum.Methods Enzymol.,105,328-331.
32. Yu, H.J.,Lin, B.R.,Lee, H.S.,Shun, C.T.,Yang, C.C.,Lai, T.Y.(2005).Sympathetic vesicovascular reflex induced by acute urinary retention evokes proinflammatory and proapoptotic injury in rat liver.Am. J. Physiol. Renal Physiol.,288,1005-1014.
33. Zhong, L.,Goldberg, M.S.,Gao, Y.T.,Jin, F.(1999).Lung cancer and indoor air pollution arising from Chinese-style cooking among nonsmoking women living in Shanghai, China.Epidemiology,5,488-494.

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