Strain Difference of DNA-Binding Activity of NF-κB and Cytokine Gene Expression in Spleen Dendritic Cells of Lewis and Fischer Rats

10.4077/CJP.2009.AMH077

Gen-Hong Yao；Lin-Song Yang；Ya-Yi Hou

dendritic cell ； NF-κB ； cytokines

The Chinese Journal of Physiology

52卷6期（2009 / 12 / 01）

451 - 454

英文

To investigate the mechanisms of different responses to inflammatory stimuli between Lewis and Fischer rats, the DNA-binding activity of the nuclear factor kappa B (NF-κB) and cytokine production of spleen dendritic cells (SDC) in Lewis and Fischer rats after peptidoglycan-polysaccharide (PG-PS) treatment were determined. The results show that the DNA-binding activities of NF-κB in SDC were higher in Lewis rats than in Fischer rats. Furthermore, in Lewis rats, the increase in NF-κB DNAbinding activities was dose-dependent. However, there is no significant change in SDC of Fischer rats. In Lewis rats, the levels of IL-2 and IL-4 were decreased along with the increase of the concentration of PG-PS while TNF-α was increased. However, there was no obvious change of cytokine expression in Fischer rats in the presence of PG-PS. In conclusion, these findings indicate that the differences in the DNA-binding activity of NF-κB and cytokine production might mediate strain-specific differences of susceptibility to chronic inflammatory stimuli in Lewis and Fischer rats.

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