Protection by 3'-Methoxypuerarin of Rat Hippocampal Neurons against Ischemia/Reperfusion Injury

10.4077/CJP.2010.AMK029

Ya-Jun Liu；Xiao-Nan Chen；Ke-Jing Liu；Qiang Chai；Jing-Xin Li；Zhi-Xiang Liu；Dong Sun；Lian-Bi Chen

3'-methoxypuerarin ； hippocampal neurons ； ischemia/reperfusion injury ； apoptosis ； rat

The Chinese Journal of Physiology

53卷2期（2010 / 04 / 01）

136 - 140

英文

3'-Methoxypuerarin (3'-MOP) is an isoflavone extracted from radix puerariae. The aim of this study was to investigate the role and the mechanism of 3'-MOP in the protection of hippocampal neurons against cerebral ischemia/reperfusion (I/R) injury in rats. I/R injury was induced by a modified fourvessel occlusion model. Rats were randomly divided into an I/R group, an I/R + 3'-MOP group and a control group. Histological changes in the neurons of the hippocampal CA1 region were observed with hematoxylin and eosine (H&E) staining. The apoptotic neurons in the hippocampal CA1 area were counted with the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining. The results showed that compared with the I/R group, 3'-MOP increased the number of surviving neurons in the hippocampal CA1 region (P＜0.001) and markedly reduced the number of apoptotic pyramidal neurons (P＜0.001) after I/R injury. In conclusion, 3'-MOP can protect hippocampal neurons against I/R injury by inhibiting apoptosis.

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