Effects of Transient Receptor Potential-Like Current on the Firing Pattern of Action Potentials in the Hodgkin-Huxley Neuron during Exposure to Sinusoidal External Voltage

10.4077/CJP.2010.AMM026

Bing-Shuo Chen；Yi-Ching Lo；Yen-Chin Liu；Sheng-Nan Wu

Hodgkin-Huxley model ； transient receptor potential TRP ； action potential ； computer simulations

The Chinese Journal of Physiology

53卷6期（2010 / 12 / 01）

423 - 429

英文

Transient receptor potential vanilloid-1 (TRPV1) channels play a role in several inflammatory and nociceptive processes. Previous work showed that magnetic electrical field-induced antinoceptive action is mediated by activation of capsaicin-sensitive sensory afferents. In this study, a modified Hodgkin-Huxley model, in which TRP-like current (I(subscript TRP)) was incorporated, was implemented to predict the firing behavior of action potentials (APs), as the model neuron was exposed to sinusoidal changes in externally-applied voltage. When model neuron is exposed to low-frequency sinusoidal voltage, increased maximal conductance of I(subscript TRP) can enhance repetitive bursts of APs accompanied by a shortening of inter-spike interval (ISI) in AP firing. The change in ISIs with number of interval is periodic with the phase-locking. In addition, increased maximal conductance of I(subscript TRP) can abolish chaotic pattern of AP firing in model neuron during exposure to high-frequency voltage. The ISI pattern is converted from irregular to constant, as maximal conductance of ITRP is increased under such high-frequency voltage. Our simulation results suggest that modulation of TRP-like channels functionally expressed in small-diameter peripheral sensory neurons should be an important mechanism through which it can contribute to the firing pattern of APs.

1. Wu, S. N.(2004).Simulations of the cardiac action potential based on the Hodgkin-Huxley kinetics with the use of Microsoft Excel spreadsheets.Chinese J. Physiol.,47,15-22.
   連結：
2. Bölcskei, K.,Helyes, Z.,Szabó, Á.,Sándor, K.,Elekes, K.,Németh, J.,Almási, R.,Pintér, E.,Pethõ, G.,Szolcsányi, J.(2005).Investigation of the role of TRPV1 receptors in acute and chronic nociceptive processes using gene-deficient mice.Pain,117,368-376.
3. Caterina, M. J.(2007).Transient receptor potential ion channels as participants in thermosensation and thermoregulation.Am. J. Physiol. Regul. Integr. Comp. Physiol.,292,R64-R76.
4. de Levie, R.(2004).Advanced Excel® for scientific data analysis.New York:Oxford University Press.
5. Ermentrout, G. B.(2002).Simulating, Analyzing, and Animating Dynamical System: A Guide to XPPAUT for Researchers and Students.Philadelphia:USA Society for Industrial and Applied Mathematics (SIAM).
6. Gunthorpe, M. J.,Benham, C. D.,Randall, A.,Davis, J. B.(2002).The diversity in the vanilloid (TRPV) receptor family of ion channels.Trends. Pharmacol. Sci.,23,183-191.
7. Han, J. S.(2003).Acupuncture: neuropeptide release produced by electrical stimulation of different frequencies.Trends Neurosci.,26,17-22.
8. Hille, B.(2001).Ion Channels of Excitable Membranes.Sunderland, MA:Sinauer Massachusetts, USA.
9. Hodgkin, A. L.,Huxley, A. F.(1952).A quantitative description of membrane current and its application to conduction and excitation in nerve.J. Physiol.,117,500-544.
10. Kanani, S.,Pumir, A.,Krinsky, V.(2008).Genetically engineered cardiac pacemaker: stem cells transfected with HCN2 gene and myocytes-a model.Phys. Lett. A,372,141-147.
11. Liu, L.,Lo, Y.,Chen, I.,Simon, S. A.(1997).The responses of rat trigeminal ganglion neurons to capsaicin and two nonpungent vanilloid receptor agonists, olvanil and glyceryl nonamide.J. Neurosci.,17,4101-4111.
12. Lo, Y. C.,Wu, J. R.,Wu, S. N.,Chen, I. J.(1997).Glyceryl nonivamide: a capsaicin derivative with cardiac calcitonin gene-related peptide releasing, K⁺ channel opening and vasorelaxant properties.J. Pharmacol. Exp. Ther.,281,253-260.
13. Lv, Y. G.(2007).Theoretical evaluation on monitoring hypothermic anesthesia by the electrical response of human skin neurons.Forsch. Ingenieurwes.,71,79-88.
14. Majumdar, S.,Sikdar, S. K.(2007).Periodicity in Na⁺ channel proper-ties alters excitability of a model neuron.Biochem. Biophys. Res. Commun.,359,908-914.
15. Sándor, K.,Helyes, Z.,Gyires, K.,Szolcsányi, J.,Lászió, J.(2007).Static magnetic field-induced anti-nociceptive effect and the involvement of capsaicin-sensitive sensory nerves in this mechanism.Life Sci.,81,97-102.
16. Wang, J.,Si, W.,Chie, Y.,Fei, X.(2008).Spike trains in Hodgkin-Huxley model and ISIs of acupuncture manipulations.Chaos, Solitons & Fractals,36,890-900.
17. Wang, Y. J.,Lin, M. W.,Lin, A. A.,Wu, S. N.(2008).Riluzole-induced block of voltage-gated Na⁺ current and activation of BKCa channels in cultured differentiated human skeletal muscle cells.Life Sci.,82,11-20.
18. Wu, S. N.,Chang, H. D.(2006).Diethyl pyrocarbonate, a histidinemodifying agent, directly stimulates activity of ATP-sensitive potassium channels in pituitary GH3 cells.Biochem. Pharmacol.,71,615-623.
19. Wu, S. N.,Chen, B. S.,Lin, M. W.,Liu, Y. C.(2008).Contribution of slowly inactivating potassium current to delayed firing of action potentials in NG108-15 neuronal cells: experimental and theoretical studies.J. Theor. Biol.,252,711-721.
20. Zhu, W.,Xu, P.,Cuascut, F. X.,Hall, A. K.,Oxford, G. S.(2007).Activin acutely sensitizes dorsal root ganglion neurons and induces hyperalgesia via PKC-mediated potentiation of transient receptor potential vanilloid I.J. Neurosci.,27,13770-13780.

1. Yang, Chia-Jung,Wu, Sheng-Nan,Lee, Yu-Chi,Chang, Tzu-Tung(2018).Stimulatory Action of Telmisartan, an Antagonist of Angiotensin II Receptor, on Voltage-Gated Na+ Current: Experimental and Theoretical Studies.中國生理學雜誌,61(1),1-13.