Tea Polyphenol-Induced Neuron-Like Differentiation of Mouse Mesenchymal Stem Cells

10.4077/CJP.2011.AMM003

Hua Liu；Wu-Jun Xue；Yu-Feng Xie；Xin-Shun Feng；Fu-Quan Huo

bone marrow mesenchymal stem cells ； tea polyphenol ； neuron-like cells ； differentiation

The Chinese Journal of Physiology

54卷2期（2011 / 04 / 01）

111 - 117

英文

Bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into neuron-like cells under appropriate conditions often involving toxic reagents that are not applicable for clinical transplantation. The present study investigated whether tea polyphenol (TP), a native nontoxic antioxidant, could induce mouse neuron-like cell differentiation of BMSCs in vitro. BMSCs, dissected from mouse femur bone marrow, were amplified in culture and treated with TP or β-mercaptoethanol (BME, control). Morphological changes were observed under light microscopy. After 12 h treatment with 50 μg/ml TP or 5 mM BME, most cells differentiated into neuron-like cells exhibiting neuronal morphological characteristics, cellular shrinkage and neurite growth. Immunocytochemistry and reverse transcription (RT)-PCR results demonstrated neuronal marker expression in the induced cells with no glial fibrillary acidic protein expression. Taken together, TP induced mouse BMSCs to differentiate into neuron-like cells in vitro. These findings provide a potential source for the treatment of various neurological diseases.

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