Effect of Delta-Opioid Receptor Over-Expression on Cortical Expression of GABA(subscript A) Receptor α1-Subunit in Hypoxia

10.4077/CJP.2011.AMM047

Yuan Feng；Xiao-Zhou He；Yi-Lin Yang；Jing-Shan Chen；Kai-Sheng Yin；Ying Xia

GABA receptor ； delta-opioid receptor ； cortex ； hypoxia

The Chinese Journal of Physiology

54卷2期（2011 / 04 / 01）

118 - 123

英文

Recent studies show that both delta-opioid receptors (DOR) and GABA receptors play a neuron-protective role in the mature cortex. Since we have observed that DOR over-expression renders the cortex more tolerant to hypoxic stress, we asked whether DOR over-expression affects GABA receptors expression in the cortex under hypoxia. As the first step, we investigated the expression of GABA(subscript A) receptor α1-subunit (GABA(subscript A) Rα1, the most abundant α-subunit of GABA receptors in the adult brain) in the mouse cortex with transgenic DOR over-expression after hypoxia. The results showed that GABA(subscript A) Rα1 expression was lower in the transgenic than wild-type cortex, suggesting that DOR over-expression induces an inhibitory effect on GABA(subscript A) receptor expression. Hypoxia for 1-3 days significantly increased GABA(subscript A) Rα1 expression in the wild-type cortex, which may be an adaptive strategy for protecting the cortex against hypoxic stress. Interestingly, such increase was not found in the transgenic cortex with DOR over-expression. This may represent an interactive regulation in the transgenic cortex to efficiently balance energy production and consumption for better adaptation to hypoxic environment. Since DOR over-expression increases cortical tolerance to hypoxia, an increase in GABA receptors expression (an energy-costing process) may not be necessary in the cortex with DOR over-expression.

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