Effect of Interleukin-6 (IL-6) on the Vascular Smooth Muscle Contraction in Abdominal Aorta of Rats with Streptozotocin-Induced Diabetes

10.4077/CJP.2011.AMM069

Wen-Bo Tang；Yu-Qin Zhou；Ting Zhao；Jing-Li Shan；Peng Sun；Ting-Ting Yang；Xin-Wen Chang；Sen Li；Paulus S. Wang；Dong-Ping Xie

abdominal aorta ； smooth muscle contraction ； interleukin-6 ； type 1 diabetes

The Chinese Journal of Physiology

54卷5期（2011 / 10 / 01）

16 - 21

英文

Patients with type 1 diabetes are at a risk of hypertension. However, the mechanisms behind the findings are not completely known. The aim of the present study was to investigate involvement of interleukin-6 (IL-6) on the contraction of abdominal aorta in rats with type 1 diabetes. IL-6 levels in the plasma of rats with streptozotocin-induced diabetes were determined by ELISA. The abdominal aorta was dissected free of fat and connective tissues and then cut into spiral rings. The endothelium-denuded strip was vertically suspended in tissue chambers containing 5 ml Krebs solution at 37℃ and bubbled continuously with 95% O2 and 5% CO2. The effects of phenylephrine (Phe) on the contractile responses of abdominal aorta were recorded. The effects of IL-6 and anti-rat IL-6 antibody on the Phe-induced response were also examined. Plasma levels of IL-6 increased time-dependently in rats with streptozotocininduced diabetes. Phe caused concentration-dependent contraction in aortic rings. Phe-induced contractions were higher in vascular strips of streptozotocin-induced diabetic rats than that of control rats. Pretreatment of vascular strips with IL-6 for 1 h did not cause contraction but enhanced the contraction in response to Phe. Treatment of the vascular strips with an anti-IL-6 antibody for 1 h decreased the Phe-induced contractions. These results suggest that IL-6 causes vascular smooth muscle contraction in abdominal aorta of rats with type 1 diabetes.

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