Role of KCa3.1 Channels in Proliferation and Migration of Vascular Smooth Muscle Cells by Diabetic Rat Serum

10.4077/CJP.2013.BAB104

Xing-Li Su；Hong Zhang；Wei Yu；Shuang Wang；Wei-Jun Zhu

intermediate-conductance Ca^(2+) activated K^+ channel ； migration ； proliferation ； smooth muscle cell

The Chinese Journal of Physiology

56卷3期（2013 / 06 / 30）

155 - 162

英文

Proliferation and migration of vascular smooth muscle cells (VSMCs) are important events in the development of diabetic atherosclerosis. Previous studies have suggested that KCa3.1 channels participate in atherosclerosis and coronary artery restenosis. In the present study, we attempted to clarify the roles of KCa3.1 channels in the proliferation and migration of VSMCs using experimental type-2 diabetes rat serum and aortic smooth muscle cells (SMC) prepared from non-diabetic rats. mRNA and protein levels and current density of KCa3.1 channels were greatly enhanced in cultured VSMCs treated with diabetic serum. In addition, diabetic serum promoted cell proliferation and migration in cultured VSMCs, and the effects were fully reversed in the cells treated with the KCa3.1 channels blocker TRAM-34. In conclusion, serum from diabetic rats increases the expression of KCa3.1 channels and promotes proliferation and migration of VSMCs to possibly participate in vascular remodeling in diabetes.

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