Glucose Transporter Type 4 Redistribution on the Membrane Induced by Insulin through Akt in Hydrocortisone Treatment in Rat Skeletal Muscles

10.4077/CJP.2015.BAD314

Chien-Min Chen；Lian Chiu；Hung-Chi Chen；Chun-Yuan Cheng；Woei-Cherng Shyu；Chii-Wen Chou；Cheng-You Lu；Chung-Tien Lin

Akt phoshphorylation ； GLUT4 ； hydrocortisone ； insulin ； skeletal muscle

The Chinese Journal of Physiology

58卷5期（2015 / 10 / 31）

294 - 301

英文

Hydrocortisone is a growth hormone frequently used in the treatment of low back pain. Hydrocortisone treatment has an anti-inflammation effect, which also inactivates glucose transporter type 4 (GLUT4) by p38 mitogen-activated protein kinase (MAPK) inhibition. Translocation of GLUT4 regulates body glucose homeostasis and muscle repair and is induced by insulin. In this study, 56 SD rats were divided into seven groups, and were treated with insulin or hydrocortisone in sedentary or exercise training groups. The muscle proteins and biochemical blood parameters were analyzed after 7 days of treatments. The results showed that the serum glucose increased in hydrocortisone treatment accompanied by GLUT4 inactivation in both the sedentary and exercise training rats. In the exercise training groups, GLUT4 was redistributed on the plasma membrane on co-treatment with insulin and hydrocortisone through Akt phosphorylation. Insulin treatment exerted a compensatory feedback effect on the GLUT4 translocation on hydrocortisone co-treatment, which was the cause of GLUT4 inactivation.

1. Lin, C.H.,Ho, C.Y.,Liu, C.S.,Lin, W.Y.,Li, C.I.,Yang, C.W.,Bau, D.T.,Li, T.C.,Lin, C.C.(2012).Influence of adiponectin gene polymorphisms on adiponectin serum level and insulin resistance index in taiwanese metabolic syndrome patients.Chinese J. Physiol.,55,405-411.
   連結：
2. Aoi, W.,Naito, Y.,Yoshikawa, T.(2013).Role of oxidative stress in impaired insulin signaling associated with exercise-induced muscle damage.Free Radic. Biol. Med.,65,1265-1672.
3. Baker, J.S.,Graham, M.R.,Davies, B.(2006).Steroid and prescription medicine abuse in the health and fitness community: a regional study.Eur. J. Intern. Med.,17,479-484.
4. Bryant, N.J.,Govers, R.,James, D.E.(2002).Regulated transport of the glucose transporter GLUT4.Nat. Rev. Mol. Cell Biol.,3,267-277.
5. Chatzitheodorou, D.,Mavromoustakos, S.,Milioti, S.(2008).The effect of exercise on adrenocortical responsiveness of patients with chronic low back pain, controlled for psychological strain.Clin. Rehabil.,22,319-328.
6. Davidson, D.,Patel, H.,Degoy, A.C.,Gershkovich, I.,Vancurova, I.,Miskolci, V.(2013).Differential effect of exogenous interleukin-10 versus glucocorticoids on gene expression and pro-inflammatory cytokine release by polymorphonuclear leukocytes and monocytes of the newly born.Am. J. Transl. Res.,5,103-115.
7. De Cock, K.J.S.,Delbeke, F.T.,Van Eenoo, P.,Desmet, N.,Roels, K.,De Backer, P.(2001).Detection and determination of anabolic steroids in nutritional supplements.J. Pharm. Biomed. Anal.,25,843-852.
8. Epstein, W.L.,Kligman, A.M.(1956).Suppression of local allergic reactions with hydrocortisone.J. Allergy,27,395-397.
9. Ernst, E.(2002).Adulteration of Chinese herbal medicines with synthetic drugs: a systematic review.J. Intern. Med.,252,107-113.
10. Feffer, H.L.(1956).Treatment of low-back and sciatic pain by the injection of hydrocortisone into degenerated intervertebral discs.J. Bone Joint Surg. Am.,38(A),585-592.
11. Geyer, H.,Parr, M.K.,Mareck, U.,Reinhart, U.,Schrader, Y.,Schänzer, W.(2004).Analysis of non-hormonal nutritional supplements for anabolic-androgenic steroids - results of an international study.Int. J. Sports Med.,25,124-129.
12. Huang, S.,Czech, M.P.(2007).The GLUT4 glucose transporter.Cell Metab.,5,237-252.
13. Hutber, C.A.,Hardie, D.G.,Winder, W.W.(1997).Electrical stimulation inactivates muscle acetyl-CoA carboxylase and increases AMP-activated protein kinase.Am. J. Physiol.,272,E262-E266.
14. Kafrouni, M.I.,Anders, R.A.,Verma, S.(2007).Hepatotoxicity associated with dietary supplements containing anabolic steroids.Clin. Gastroenterol. Hepatol.,5,809-812.
15. Kaminsky, P.,Walker, P.M.,Deibener, J.,Barbe, F.,Jeannesson, E.,Escanye, J.M.,Dousset, B.,Klein, M.(2012).Growth hormone potentiates thyroid hormone effects on post-exercise phosphocreatine recovery in skeletal muscle.Growth Horm. IGF Res.,22,240-244.
16. Kimura, H.,Li, X.,Torii, K.,Okada, T.,Kamiyama, K.,Mikami, D.,Kasuno, K.,Takahashi, N.,Yoshida, H.(2011).Glucocorticoid enhances hypoxia- and/or transforming growth factor-β-induced plasminogen activator inhibitor-1 production in human proximal renal tubular cells.Clin. Exp. Nephrol.,15,34-40.
17. Kurth-Kraczek, E.J.,Hirshman, M.F.,Goodyear, L.J.,Winder, W.W.(1999).5' AMP-activated protein kinase activation causes GLUT4 translocation in skeletal muscle.Diabetes,48,1667-1671.
18. Langmayr, J.J.,Obwegeser, A.A.,Schwarz, A.B.,Laimer, I.,Ulmer, H.,Ortler, M.(1995).Intrathecal steroids to reduce pain after lumbar disc surgery: a double-blind, placebo-controlled prospective study.Pain,62,357-361.
19. Lentjes, E.G.W.M.,Griep, E.N.,Boersma, J.W.,Romijn, F.P.T.H.M.,de Kloet, E.R.(1997).Glucocorticoid receptors, fibromyalgia and low back pain.Psychoneuroendocrinology,22,603-614.
20. Montessuit, C.,Rosenblatt-Velin, N.,Papageorgiou, I.,Campos, L.,Pellieux, C.,Palma, T.,Lerch, R.(2004).Regulation of glucose transporter expression in cardiac myocytes: p38 MAPK is a strong inducer of GLUT4.Cardiovasc. Res.,64,94-104.
21. Neuberger, T.J.,Kalimi, O.,Regelson, W.,Kalimi, M.,De Vries, G.H.(1994).Glucocorticoids enhance the potency of Schwann cell mitogens.J. Neurosci. Res.,38,300-313.
22. Nozaki, S.,Takeda, T.,Kitaura, T.,Takenaka, N.,Kataoka, T.,Satoh, T.(2013).Akt2 regulates Rac1 activity in the insulin-dependent signaling pathway leading to GLUT4 translocation to the plasma membrane in skeletal muscle cells.Cell. Signal.,25,1361-1371.
23. O'Fallon, K.S.,Kaushik, D.,Michniak-Kohn, B.,Dunne, C.P.,Zambraski, E.J.,Clarkson, P.M.(2012).Effects of quercetin supplementation on markers of muscle damage and inflammation after eccentric exercise.Int. J. Sport Nutr. Exerc. Metab.,22,430-437.
24. Piroli, G.G.,Grillo, C.A.,Reznikov, L.R.,Adams, S.,McEwen, B.S.,Charron, M.J.,Reagan, L.P.(2007).Corticosterone impairs insulin-stimulated translocation of GLUT4 in the rat hippocampus.Neuroendocrinology,85,71-80.
25. Ponticos, M.,Lu, Q.L.,Morgan, J.E.,Hardie, D.G.,Partridge, T.A.,Carling, D.(1998).Dual regulation of the AMP-activated protein kinase provides a novel mechanism for the control of creatine kinase in skeletal muscle.EMBO J.,17,1688-1699.
26. Sarvestani, A.S.,Amini, S.,Kalhor, M.,Roshanravan, R.,Mohammadi, M.,Lebaschi, A.H.(2013).Intraperitoneal hydrocortisone for pain relief after laparoscopic cholecystectomy.Saudi J. Anaesth.,7,14-17.
27. Shen, Y.,Zhao, Y.,Zheng, D.,Chang, X.,Ju, S.,Guo, L.(2013).Effects of orexin A on GLUT4 expression and lipid content via MAPK signaling in 3T3-L1 adipocytes.J. Steroid Biochem. Mol. Biol.,138,376-383.
28. Stuhlmeier, K.M.,Pollaschek, C.(2004).Glucocorticoids inhibit induced and non-induced mRNA accumulation of genes encoding hyaluronan synthases (HAS): hydrocortisone inhibits HAS1 activation by blocking the p38 mitogen-activated protein kinase signalling pathway.Rheumatology,43,164-169.
29. Sudhaus, S.,Mollenberg, T.,Plaas, H.,Willburger, R.,Schmieder, K.,Hasenbring, M.(2012).Cortisol awakening response and painrelated fear-avoidance versus endurance in patients six months after lumbar disc surgery.Appl. Psychophysiol. Biofeedback,37,121-130.
30. Wang, Q.,Somwar, R.,Bilan, P.J.,Liu, Z.,Jin, J.,Woodgett, J.R.,Klip, A.(1999).Protein kinase B/Akt participates in GLUT4 translocation by insulin in L6 myoblasts.Mol. Cell. Biol.,19,4008-4018.
31. Yang, J.(2010).Role of clusters in insulin-regulated GLUT4 trafficking in adipose cells: a new paradigm?.Int. J. Biol. Sci.,6,716-718.

1. Yang, Shih-Hsing,Wang Hsu, Guoo-Shyng,Peng, Shih-Chi,Lin, Jau-Chen,Jung, Fang,Jow, Guey-Mei(2016).Effects of Hydrocortisone on Activation of Inflammation via Interleukin-33 in Ventilator-Induced Lung Injury.中國生理學雜誌,59(5),284-292.