题名

Roles of NAD in Protection of Axon against Degeneration via SIRT1 Pathways

DOI

10.4077/CJP.2016.BAD331

作者

Jing Zhang；Wei-Hua Guo；Xiao-Xia Qi；Gui-Bao Li；Yan-Lai Hu；Qi Wu；Zhao-Xi Ding；Hong-Yu Li；Jing Hao；Jin-Hao Sun

关键词

axonal degeneration ； DRG ； NAD ； protection ； SIRT ； vincristine

期刊名称

The Chinese Journal of Physiology

卷期/出版年月

59卷2期（2016 / 04 / 30）

页次

78 - 86

内容语文

英文
英文摘要

Axonal degeneration is a common pathological change of neurogenical disease which often arises before the neuron death. But it had not found any effective method to protect axon from degeneration. In this study we intended to confirm the protective effect of nicotinamide adenine dinucleotide (NAD), investigate the optimal administration dosage and time of NAD, and identify the relationship between silence signal regulating factor 1 (SIRT1) and axonal degeneration. An axonal degeneration model was established using dorsal root ganglion (DRG) neurons injured by vincristine to observe the protective effects of NAD to the injured axons. In addition, the potential contribution of the SIRT1 in axonal degeneration was also investigated. Through the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, immunochemistry staining, axons counting and length measuring, transmission electron microscope (TEM) observation, we demonstrated that NAD played an important role in preventing axonal degeneration. Further study revealed that the expression of SIRT1 and phosphorylated Akt1 (p-Akt1) was up-regulated when NAD was added into the culturing medium. Taking together, our results demonstrated that NAD might delay the axonal degeneration through SIRT1/Akt1 pathways.
主题分类 醫藥衛生 > 基礎醫學
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