题名

Vasodilatory Effects of Aloperine in Rat Aorta and Its Possible Mechanisms

DOI

10.4077/CJP.2018.BAH609

作者

Caiyan Yang；Yang Yu；Fan Wu；Yuhua Wu；Jun Feng；Lin Yan；Liangjuan Han；Jun Ren；Lihong Nie；Ru Zhou

关键词

aloperine ； ATP-sensitive potassium channels ； endothelium independent ； vasodilation ； voltage-operated potassium channels

期刊名称

The Chinese Journal of Physiology

卷期/出版年月

61卷5期（2018 / 10 / 31）

页次

293 - 301

内容语文

英文
中文摘要

The aim of this study was to investigate the vasodilatory effects of aloperine, one of main alkaloid was extracted from Sophora alopecuroides, on rat isolated thoracic aortic rings and its possible mechanisms. The isolated aortic arteries from normotensive Sprague Dawley rats were precontracted with phenylephrine (1 × 10^(-6) M) or KCl (60 mM). Then, aloperine (3.44 × 10^(-3) -17.21 × 10^(-3) M) was added cumulatively and the tension curves was observed and recorded. The changes in tension in both endothelium-intact and endothelium-denuded aortic rings were also recorded. Afterwards, the interaction between aloperine with NG-nitro-L-arginine methylester (0.1 mM), indomethacin (1 × 10^(-3) mM), tetraethylammonium (10 mM), 4-aminopyridine (5 mM), BaCl_2 (1 mM) and glibenclamide (0.01 mM) was evaluated. In this study, aloperine caused concentration-dependent relaxations in aortic rings precontracted with phenylephrine, but this effect was not observed in KCl-pretreated rings. Removal of endothelium showed no influence on vasodilatory effects of aloperine. In addition, preincubation with NG-nitro-L-arginine methylester and indomethacin did not inhibit the vasodilatory effects of aloperine, suggesting that the vasodilative action is endothelium-independent. Relaxant responses to aloperine were inhibited by tetraethylammonium and 4-aminopyridine. However, the vasorelaxant effect of aloperine was also not influenced by the preincubation with BaCl_2 and glibenclamide. These findings suggest that aloperine-induced vasorelaxation effects are mainly due to the operations of voltage-operated potassium channels and ATP-sensitive potassium channels.
主题分类 醫藥衛生 > 基礎醫學
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