Effects of Ganoderma lucidum and 'Essence of Chicken' on Physical Fatigue Recovery and Exercise Performance Improvement

10.4077/CJP.2018.BAH646

HuaShuai Li；Ying-Ju Chen；Yi-Ju Hsu；Ming-Fang Wu；Chien-Chao Chiu；Yu-Tang Tung；Wei-Jern Tsai；Wen-Ching Huang；Chi-Chang Huang

clearance rate ； essence of chicken ； exercise fatigue ； physical activities ； safety

The Chinese Journal of Physiology

61卷6期（2018 / 12 / 31）

372 - 383

英文

A fast-paced lifestyle, pressure from the environment and a heavy work load often cause extreme tiredness in modern life. Different kinds of nutritional supplements in the form of functional foods or traditional Chinese medicine, such as 'essence of chicken' and Ganoderma lucidum have been claimed to benefit physical performance and promote health. Previous studies have revealed that 'essence of chicken' or G. lucidum have a wide spectrum of biological activities. In this study, we combined these two ingredients together (designated as CEG) to evaluate their synergistic effects on physiological adaption on exercise fatigue and physical activities. The ICR strain mice were allocated as 0, 833, 1666, and 4165 mg/kg dose groups and administrated by oral gavage consecutively for 4 weeks. Physical activities including grip strength and aerobic endurance were evaluated. Various fatigue-associated biochemical variables such as lactate, BUN or CK were also evaluated. The levels of liver and muscle glycogen were measured as an indicator of energy storage at the end of the experiment. Safety assessments for supplementation were also evaluated. CEG supplementation significantly increased the endurance and grip strength and demonstrated beneficial effects on lactate production and clearance rate after an acute exercise challenge. The CEG supplementation significantly mitigated the BUN and CK indexes after extended exercise and elevated the glycogen content in the liver and muscle tissues. According to body composition, biochemical and histopathological data, daily administration of CEG for over 28 days (subacute toxicity) also demonstrated reasonable safety results for supplementation. Combined G. lucidum and 'essence of chicken' can significantly increase the exercise performance and improve fatigue recovery. It may also provide a viable alternative nutritional supplement for health promotion.

1. Lo, H.I.,Tsi, D.,Tan, A.C.,Wang, S.W.,Hsu, M.C.(2005).Effects of postexercise supplementation of chicken essence on the elimination of exercise-induced plasma lactate and ammonia.Chinese J. Physiol.,48,187-192.
   連結：
2. Su, W.L.,Huang, W.C.,Chen, I.N.,Chen, W.C.,Huang, C.C.,Huang, C.H.(2015).Investigation of whole-body vibration training on physiological and biochemical characteristics in mice.Phys. Educ. J.,48,33-44.
   連結：
3. Asmussen, E.(1979).Muscle fatigue.Med. Sci. Sports,11,313-321.
4. Bergstrom, J.,Hermansen, L.,Hultman, E.,Saltin, B.(1967).Diet, muscle glycogen and physical performance.Acta Physiol. Scand.,71,140-150.
5. Boh, B.,Berovic, M.,Zhang, J.,Zhi-Bin, L.(2007).Ganoderma lucidum and its pharmaceutically active compounds.Biotechnol. Annu. Rev.,13,265-301.
6. Boldyrev, A.A.,Stvolinskiĭ, S.L.,Fedorova, T.N.(2007).Carnosine: endogenous physiological corrector of antioxidative system activity.Usp. Fiziol. Nauk.,38,57-71.
7. Brown, R.F.,Schutte, N.S.(2006).Direct and indirect relationships between emotional intelligence and subjective fatigue in university students.J. Psychosom. Res.,60,585-593.
8. Cairns, S.P.(2006).Lactic acid and exercise performance: Culprit or friend?.Sports Med.,36,279-291.
9. Castro, B.,Kuang, S.(2017).Evaluation of muscle performance in mice by treadmill exhaustion test and whole-limb grip strength assay.Bio Protoc.,7,e2237.
10. Chen, W.C.,Huang, W.C.,Chiu, C.C.,Chang, Y.K.,Huang, C.C.(2014).Whey protein improves exercise performance and biochemical profiles in trained mice.Med. Sci. Sports Exerc.,46,1517-1524.
11. Chiu, C.C.,Wang, Y.C.,Huang, W.C.,Chen, Y.H.,Hung, S.W.,Huang, Y.T.,Chuang, H.L.,Chang, Y.C.(2017).Differences in genetic background contribute to Pseudomonas exotoxin a-induced hepatotoxicity in rats.Toxins,9,224.
12. Collado Mateo, D.,Pazzi, F.,Domínguez Muñoz, F.J.,Martín Martínez, J.P.,Olivares, P.R.,Gusi, N.,Adsuar, J.C.(2015).Ganoderma lucidum improves physical fitness in women with fibro-myalgia.Nutr. Hosp.,32,2126-2135.
13. Conner, J.D.,Wolden-Hanson, T.,Quinn, L.S.(2014).Assessment of murine exercise endurance without the use of a shock grid: an alternative to forced exercise.J. Vis. Exp.,90,51846.
14. Gill, S.K.,Rieder, M.J.(2008).Toxicity of a traditional Chinese medicine, Ganoderma lucidum, in children with cancer.Can. J. Clin. Pharmacol.,15,e275-e285.
15. He, H.,He, J.P.,Sui, Y.J.,Zhou, S.Q.,Wang, J.(2008).The hepato-protective effects of Ganoderma lucidum peptides against carbon tetrachloride-induced liver injury in mice.J. Food Biochem.,32,628-641.
16. Hsiao, C.Y.,Chen, Y.M.,Hsu, Y.J.,Huang, C.C.,Sung, H.C.,Chen, S.S.(2017).Supplementation with Hualian No. 4 wild bitter gourd (Momordica charantia Linn. var. abbreviata ser.) extract increases anti-fatigue activities and enhances exercise performance in mice.J. Vet. Med. Sci.,79,1110-1119.
17. Huang, W.C.,Chang, Y.C.,Chen, Y.M.,Hsu, Y.J.,Huang, C.C.,Kan, N.W.,Chen, S.S.(2017).Whey protein improves marathoninduced injury and exercise performance in elite track runners.Int. J. Med. Sci.,14,648-654.
18. Huang, W.C.,Hsu, Y.J.,Wei, L.,Chen, Y.J.,Huang, C.C.(2016).Association of physical performance and biochemical profile of mice with intrinsic endurance swimming.Int. J. Med. Sci.,13,892-901.
19. Huang, W.C.,Huang, C.C.,Chuang, H.L.,Chen, W.C.,Hsu, M.C.(2017).Cornu cervi pantotrichum supplementation improves physiological adaptions during intensive endurance training.J. Vet. Med. Sci.,79,674-682.
20. Huang, W.C.,Lin, C.I.,Chiu, C.C.,Lin, Y.T.,Huang, W.K.,Huang, H.Y.,Huang, C.C.(2014).Chicken essence improves exercise performance and ameliorates physical fatigue.Nutrients,6,2681-2696.
21. Kim, H.,Park, S.,Han, D.S.,Park, T.(2003).Octacosanol supplementation increases running endurance time and improves biochemical parameters after exhaustion in trained rats.J. Med. Food,6,345-351.
22. Kirkendall, D.T.(1990).Mechanisms of peripheral fatigue.Med. Sci. Sports Exerc.,22,444-449.
23. Koch, LG,Britton, SL.(2001).Artificial selection for intrinsic aerobic endurance running capacity in rats.Physiol. Genomics,5,45-52.
24. Konagai, C.,Watanabe, H.,Abe, K.,Tsuruoka, N.,Koga, Y.(2013).Effects of essence of chicken on cognitive brain function: a nearinfrared spectroscopy study.Biosci. Biotechnol. Biochem.,77,178-181.
25. Li, Y.F.,He, R.R.,Tsoi, B.,Kurihara, H.(2012).Bioactivities of chicken essence.J. Food Sci.,77,105-110.
26. Meeusen, R.,Watson, P.,Hasegawa, H.,Roelands, B.,Piacentini, M.F.(2006).Central fatigue: the serotonin hypothesis and beyond.Sports Med.,36,881-909.
27. Mettlach, G.,Polo-Parada, L.,Peca, L.,Rubin, C.T.,Plattner, F.,Bibb, J.A.(2014).Enhancement of neuromuscular dynamics and strength behavior using extremely low magnitude mechanical signals in mice.J. Biomech.,47,162-167.
28. Nielsen, J.,Ortenblad, N.(2013).Physiological aspects of the subcellular localization of glycogen in skeletal muscle.Appl. Physiol. Nutr. Metab.,38,91-99.
29. Ouyang, M.Z.,Lin, L.Z.,Lv, W.J.,Zuo, Q.,Lv, Z.,Guan, J.S.,Wang, S.T.,Sun, L.L.,Chen, H.R.,Xiao, Z.W.(2016).Effects of the polysaccharides extracted from Ganoderma lucidum on chemo-therapy-related fatigue in mice.Int. J. Biol. Macromol.,91,905-910.
30. Ploegmakers, J.J.,Hepping, A.M.,Geertzen, J.H.,Bulstra, S.K.,Stevens, M.(2013).Grip strength is strongly associated with height, weight and gender in childhood: a cross sectional study of 2241 children and adolescents providing reference values.J. Physiother.,59,255-261.
31. Sanchez-Delgado, G.,Martinez-Tellez, B.,Olza, J.,Aguilera, C.M.,Gil, Á.,Ruiz, J.(2015).Role of exercise in the activation of brown adipose tissue.Ann. Nutr. Metab.,67,21-32.
32. Shi, Y.,Cai, D.,Wang, X.,Liu, X.(2012).Immunomodulatory effect of Ganoderma lucidum polysaccharides (GLP) on long-term heavy-load exercising mice.Int. J. Vitam. Nutr. Res.,82,383-390.
33. Sim, M.K.(2001).Cardiovascular actions of chicken-meat extract in normo- and hypertensive rats.Brit. J. Nutr.,86,97-103.
34. Skenderi, K.P.,Kavouras, S.A.,Anastasiou, C.A.,Yiannakouris, N.,Matalas, A.L.(2006).Exertional Rhabdomyolysis during a 246-km continuous running race.Med. Sci. Sports Exerc.,38,1054-1057.
35. Smina, T.P.,Mathew, J.,Janardhanan, K.K.,Devasagayam, T.P.(2011).Antioxidant activity and toxicity profile of total triterpenes isolated from Ganoderma lucidum (Fr.) P. Karst occurring in South India.Environ. Toxicol. Pharmacol.,32,438-446.
36. Sun, L.,Wei Jie Tan, K.,Jeyakumar Henry, C.(2015).Co-ingestion of essence of chicken to moderate glycaemic response of bread.Int. J. Food Sci. Nutr.,66,931-935.
37. Tsi, D.,Nah, A.K.,Kiso, Y.,Moritani, T.,Ono, H.(2003).Clinical study on the combined effect of capsaicin, green tea extract and essence of chicken on body fat content in human subjects.J. Nutr. Sci. Vitaminol.,49,437-441.
38. Wang, F.,Zhou, Z.,Ren, X.,Wang, Y.,Yang, R.,Luo, J.,Strappe, P.(2015).Effect of Ganoderma lucidum spores intervention on glucose and lipid metabolism gene expression profiles in type 2 diabetic rats.Lipids Health Dis.,14,49.
39. Williams, C.,Rollo, I.(2015).Carbohydrate nutrition and team sport performance.Sports Med.,45,S13-S22.
40. Wu, H.C.,Pan, B.S.,Chang, C.L.,Shiau, C.Y.(2005).Low-molecular-weight peptides as related to antioxidant properties of chicken essence.J. Food Drug Anal.,13,176-183.
41. Wu, T.,Yao, C.,Tsang, F.,Huang, L.,Zhang, W.,Jiang, J.,Mao, Y.,Shao, Y.,Kong, B.,Singh, P.,Fu, Z.(2015).Facilitated physiological adaptation to prolonged circadian disruption through dietary supplementation with essence of chicken.Chronobiol. Int.,32,1458-1468.
42. Wu, X.,Zeng, J.,Hu, J.,Liao, Q.,Zhou, R.,Zhang, P.,Chen, Z.(2013).Hepatoprotective effects of aqueous extract from Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (higher basidiomycetes) on α-amanitin-induced liver injury in mice.Int. J. Med. Mushrooms,15,383-391.
43. Yamano, E.,Tanaka, M.,Ishii, A.,Tsuruoka, N.,Abe, K.,Watanabe, Y.(2013).Effects of chicken essence on recovery from mental fatigue in healthy males.Med. Sci. Monit.,19,540-547.
44. Yamano, T.,Niijima, A.,Iimori, S.,Tsuruoka, N.,Kiso, Y.,Nagai, K.(2001).Effect of L-carnosine on the hyperglycemia caused by intracranial injection of 2-deoxy-D-glucose in rats.Neurosci. Lett.,313,78-82.
45. Young, H.,Benton, D.,Carter, N.(2015).The effect of chicken extract on mood, cognition and heart rate variability.Nutrients,7,887-904.
46. Zhao, H.,Zhang, Q.,Zhao, L.,Huang, X.,Wang, J.,Kang, X.(2012).Spore powder of Ganoderma lucidum improves cancer-related fatigue in breast cancer patients undergoing endocrine therapy: a pilot clinical trial.Evid. Based Complement. Alternat. Med.,2012,809614.
47. Zhonghui, Z.,Xiaowei, Z.,Fang, F.(2014).Ganoderma lucidum polysaccharides supplementation attenuates exercise-induced oxidative stress in skeletal muscle of mice.Saudi J. Biol. Sci.,21,119-123.