Sex Differences in Antiaging Response to Short-and Long-Term High-Intensity Interval Exercise in Rat Cardiac Muscle: Telomerase Activity, Total Antioxidant/Oxidant Status

10.4103/CJP.CJP_52_19

Marziyeh Saghebjoo；Saber Sadeghi-Tabas；Iman Saffari；Azin Ghane；Ivan Dimauro

Cardiac muscle ； high-intensity interval exercise ； sex differences ； telomerase activity ； total antioxidant/oxidant status

The Chinese Journal of Physiology

62卷6期（2019 / 12 / 31）

261 - 266

英文

Cardiovascular disease prevails with age which varies according to sex. Telomere length plays an important role in aging. Despite the great benefits of high-intensity interval training (HIIT), the acute responses to HIIT with different intervals have not been elucidated in different sexes. This study was conducted to investigate the sex-dependent responses of telomerase enzyme activity, total oxidant status (TOS), total antioxidant capacity (TAC), and the ratio of TAC/TOS to short-.and long-term high-intensity interval exercise (HIIE) in cardiac muscle of male and female rats. Forty adult Wistar rats were randomly allocated to six groups: male and female HIIE with short-term intervals (MHIIESh and FHIIESh, respectively), male and female HIIE with long-term intervals (MHIIEL and FHIIEL, respectively), and controls groups. Telomerase activity, TAC, and TOS levels were examined immediately after exercise in the cardiac muscle. The level of telomerase enzyme activity, TOS level, and the ratio of TAC/TOS did not change after HIIE with short-term interval and HIIE with long-term interval (HIIEL) in male and female rats (P=0.52, 0.69, and 0.08, respectively). There was a statistically significant decrease in the TAC level in the MHIIESh group (P=0.04). Furthermore, a significant decrease was observed in the HIIEL in both male and female rats (P=0.03 and 0.04, respectively). Acute exposure to HIIE with short-and long-term intervals would not result in a significant change in some indicators of biological aging. However, due to gender-specific biological differences, further studies will provide evidence regarding the roles of HIIE at different times of intervals, which contribute to aging prevention.

1. Balcı, SS,Pepe, H(2012).Effects of gender, endurance training and acute exhaustive exercise on oxidative stress in the heart and skeletal muscle of the rat.Chin J Physiol,55,236-244.
   連結：
2. Aix, E,Gallinat, A,Flores, I(2018).Telomeres and telomerase in heart regeneration.Differentiation,100,26-30.
3. Arsenis, NC,You, T,Ogawa, EF,Tinsley, GM,Zuo, L(2017).Physical activity and telomere length: Impact of aging and potential mechanisms of action.Oncotarget,8,45008-45019.
4. Azhar, S,Cao, L,Reaven, E(1995).Alteration of the adrenal antioxidant defense system during aging in rats.J Clin Invest,96,1414-1424.
5. Barrett, EL,Richardson, DS(2011).Sex differences in telomeres and lifespan.Aging Cell,10,913-921.
6. Bernardes de Jesus, B,Vera, E,Schneeberger, K,Tejera, AM,Ayuso, E,Bosch, F(2012).Telomerase gene therapy in adult and old mice delays aging and increases longevity without increasing cancer.EMBO Mol Med,4,691-704.
7. Blasco, MA(2005).Telomeres and human disease: Ageing, cancer and beyond.Nat Rev Genet,6,611-622.
8. Bloomer, RJ,Goldfarb, AH,Wideman, L,McKenzie, MJ,Consitt, LA(2005).Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress.J Strength Cond Res,19,276-285.
9. Borghini, A,Giardini, G,Tonacci, A,Mastorci, F,Mercuri, A,Mrakic-Sposta, S(2015).Chronic and acute effects of endurance training on telomere length.Mutagenesis,30,711-716.
10. Cachofeiro, V,Goicochea, M,de Vinuesa, SG,Oubiña, P,Lahera, V,Luño, J(2008).Oxidative stress and inflammation, a link between chronic kidney disease and cardiovascular disease.Kidney Int Suppl,74,S4-S9.
11. Chilton, WL,Marques, FZ,West, J,Kannourakis, G,Berzins, SP,O’Brien, BJ(2014).Acute exercise leads to regulation of telomere-associated genes and microRNA expression in immune cells.PLoS One,9,e92088.
12. Cipryan, L(2018).The effect of fitness level on cardiac autonomic regulation, IL-6, total antioxidant capacity, and muscle damage responses to a single bout of high-intensity interval training.J Sport Health Sci,7,363-371.
13. Colafella, KM,Denton, KM(2018).Sex specific differences in hypertension and associated cardiovascular disease.Nat Rev Nephrol,14,185-201.
14. Denham, J,Nelson, CP,O’Brien, BJ,Nankervis, SA,Denniff, M,Harvey, JT(2013).Longer leukocyte telomeres are associated with ultra-endurance exercise independent of cardiovascular risk factors.PLoS One,8,e69377.
15. Epel, ES,Blackburn, EH,Lin, J,Dhabhar, FS,Adler, NE,Morrow, JD(2004).Accelerated telomere shortening in response to life stress.Proc Natl Acad Sci U S A,101,17312-17315.
16. Gardner, M,Bann, D,Wiley, L,Cooper, R,Hardy, R,Nitsch, D(2014).Gender and telomere length: Systematic review and meta-analysis.Exp Gerontol,51,15-27.
17. Goto, C,Nishioka, K,Umemura, T,Jitsuiki, D,Sakagutchi, A,Kawamura, M(2007).Acute moderate-intensity exercise induces vasodilation through an increase in nitric oxide bioavailiability in humans.Am J Hypertens,20,825-830.
18. Hakim, AA,Petrovitch, H,Burchfiel, CM,Ross, GW,Rodriguez, BL,White, LR(1998).Effects of walking on mortality among nonsmoking retired men.N Engl J Med,338,94-99.
19. Londoño-Vallejo, JA,Wellinger, RJ(2012).Telomeres and telomerase dance to the rhythm of the cell cycle.Trends Biochem Sci,37,391-399.
20. Lu, K,Wang, L,Wang, C,Yang, Y,Hu, D,Ding, R(2015).Effects of high-intensity interval versus continuous moderate-intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model.Mol Med Rep,12,2374-2382.
21. Lubkowska, A,Bryczkowska, I,Gutowska, I,Rotter, I,Marczuk, N,Baranowska-Bosiacka, I(2019).The effects of swimming training in cold water on antioxidant enzyme activity and lipid peroxidation in erythrocytes of male and female aged rats.Int J Environ Res Public Health,16,E647.
22. Ludlow, A.(2011).Telomere Dynamics and Regulation: Effects of Chronic Exercise, Acute Exercise, and Oxidative Stress.College Park:University of Maryland.
23. Ludlow, AT,Gratidão, L,Ludlow, LW,Spangenburg, EE,Roth, SM(2017).Acute exercise activates p38 MAPK and increases the expression of telomere-protective genes in cardiac muscle.Exp Physiol,102,397-410.
24. Ludlow, AT,Ludlow, LW,Roth, SM(2013).Do telomeres adapt to physiological stress? Exploring the effect of exercise on telomere length and telomere-related proteins.Biomed Res Int,2013
25. Majerská, J,Sýkorová, E,Fajkus, J(2011).Non-telomeric activities of telomerase.Mol Biosyst,7,1013-1023.
26. Manson, JE,Greenland, P,LaCroix, AZ,Stefanick, ML,Mouton, CP,Oberman, A(2002).Walking compared with vigorous exercise for the prevention of cardiovascular events in women.N Engl J Med,347,716-725.
27. Ornish, D,Lin, J,Daubenmier, J,Weidner, G,Epel, E,Kemp, C(2008).Increased telomerase activity and comprehensive lifestyle changes: A pilot study.Lancet Oncol,9,1048-1057.
28. Parker, L,McGuckin, TA,Leicht, AS(2014).Influence of exercise intensity on systemic oxidative stress and antioxidant capacity.Clin Physiol Funct Imaging,34,377-383.
29. Pingitore, A,Lima, GP,Mastorci, F,Quinones, A,Iervasi, G,Vassalle, C(2015).Exercise and oxidative stress: Potential effects of antioxidant dietary strategies in sports.Nutrition,31,916-922.
30. Pirmoradi, S,Fathi, E,Farahzadi, R,Pilehvar-Soltanahmadi, Y,Zarghami, N(2018).Curcumin affects adipose tissue-derived mesenchymal stem cell aging through TERT gene expression.Drug Res,68,213-221.
31. Puterman, E,Lin, J,Blackburn, E,O’Donovan, A,Adler, N,Epel, E(2010).The power of exercise: Buffering the effect of chronic stress on telomere length.PLoS One,5,e10837.
32. Qian, X,Asad, SB,Li, J,Wang, J,Wei, D,Zhao, Y(2019).Link between cardiac function and the antioxidative defense mechanism in aged rats.Biochem Biophys Res Commun,513,1100-1105.
33. Ramos, D,Martins, EG,Viana-Gomes, D,Casimiro-Lopes, G,Salerno, VP(2013).Biomarkers of oxidative stress and tissue damage released by muscle and liver after a single bout of swimming exercise.Appl Physiol Nutr Metab,38,507-511.
34. Rebelo-Marques, A,De Sousa Lages, A,Andrade, R,Ribeiro, CF,Mota-Pinto, A,Carrilho, F(2018).Aging hallmarks: The benefits of physical exercise.Front Endocrinol,9,258.
35. Rehkopf, DH,Needham, BL,Lin, J,Blackburn, EH,Zota, AR,Wojcicki, JM(2016).Leukocyte telomere length in relation to 17 biomarkers of cardiovascular disease risk: A cross-sectional study of US adults.PLoS Med,13,e1002188.
36. Richardson, GD,Breault, D,Horrocks, G,Cormack, S,Hole, N,Owens, WA(2012).Telomerase expression in the mammalian heart.FASEB J,26,4832-4840.
37. Shammas, MA(2011).Telomeres, lifestyle, cancer, and aging.Curr Opin Clin Nutr Metab Care,14,28-34.
38. Stewart, KJ(2002).Exercise training and the cardiovascular consequences of type 2 diabetes and hypertension: Plausible mechanisms for improving cardiovascular health.JAMA,288,1622-1631.
39. Syslová, K,Böhmová, A,Mikoška, M,Kuzma, M,Pelclová, D,Kačer, P(2014).Multimarker screening of oxidative stress in aging.Oxid Med Cell Longev,2014
40. Tucker, WJ,Sawyer, BJ,Jarrett, CL,Bhammar, DM,Gaesser, GA(2015).Physiological responses to high-intensity interval exercise differing in interval duration.J Strength Cond Res,29,3326-3335.
41. Werner, C,Fürster, T,Widmann, T,Pöss, J,Roggia, C,Hanhoun, M(2009).Physical exercise prevents cellular senescence in circulating leukocytes and in the vessel wall.Circulation,120,2438-2447.
42. Werner, C,Hanhoun, M,Widmann, T,Kazakov, A,Semenov, A,Pöss, J(2008).Effects of physical exercise on myocardial telomere-regulating proteins, survival pathways, and apoptosis.J Am Coll Cardiol,52,470-482.
43. Xie, Z,Jay, KA,Smith, DL,Zhang, Y,Liu, Z,Zheng, J(2015).Early telomerase inactivation accelerates aging independently of telomere length.Cell,160,928-939.
44. Xin, H,Liu, D,Songyang, Z(2008).The telosome/shelterin complex and its functions.Genome Biol,9,232.
45. Yamamoto, T,Ohkuwa, T,Itoh, H,Sato, Y,Naoi, M(2002).Effect of gender differences and voluntary exercise on antioxidant capacity in rats.Comp Biochem Physiol C Toxicol Pharmacol,132,437-444.
46. Yip, BW,Mok, HO,Peterson, DR,Wan, MT,Taniguchi, Y,Ge, W(2017).Sex-dependent telomere shortening, telomerase activity and oxidative damage in marine medaka Oryzias melastigma during aging.Mar Pollut Bull,124,701-709.
47. Zietzer, A,Buschmann, EE,Janke, D,Li, L,Brix, M,Meyborg, H(2017).Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.Acta Physiol,220,251-262.