Primulina rufipes, a new species of Gesneriaceae from Guangxi, China

10.6165/tai.2022.67.40

Yu-Lan SU；Zhang-Ping HUANG；Ping YANG；Wei-Bin XU；Yan LIU

Karst cave ； limestone flora ； morphology ； molecular phylogeny ； Primulina gongchengensis ； P. huijiensis ； P. yulinensis

TAIWANIA

67卷1期（2022 / 03 / 01）

40 - 46+s1-5

英文

Primulina rufipes Y.L. Su, P. Yang & Yan Liu (Gesneriaceae), a new species from Guangxi, China, is described and illustrated. Molecular phylogenetic analyses unambiguously put this species in Primulina. Morphological observations and comparisons showed that P. rufipes is similar to P. huaijiensis Z.L. Ning & J. Wang, differing by all over the plant densely pubescent and glandular-puberulent, leaf blade elliptic to rhomboid-ovate, and obliquely campanulate corolla without appendages. P. rufipes is also similar to P. yulinensis Ying Qin & Yan Liu and P. gongchengensis Y.S. Huang & Yan Liu in vegetative characters, but its obliquely campanulate corolla is obviously different from the latter two species.

1. Edgar, R.-C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32(5): 1792–1797.
   連結：
2. Guo, J., B. Pan, J. Liu, W.-B. Xu and K.-F. Chung. 2015. Three new species of Primulina (Gesneriaceae) from limestone karsts of China based on morphological and molecular evidence. Bot. Stud. 56(1): 34–46.
   連結：
3. Liu, Yan, W.-B. Xu and B. Pan. 2010. Wentsaiboea tiandengensis sp. nov. and W. luochengensis sp. nov. (Gesneriaceae) from Karst caves in Guangxi, southern China. Nordic J. Bot. 28(6): 739–745.
   連結：
4. Möller, M., M. Pfosser, C.-G. Jang, V. Mayer, A. Clark, M.-L. Hollingsworth, M.-H.-J. Barfuss, Y.-Z. Wang, M. Kiehn and A. Weber. 2009. A preliminary phylogeny of the Didymocarpoid Gesneriaceae based on three molecular data sets: incon gruence with available tribal classifications. Amer. J. Bot. 96(5): 989–1010.
   連結：
5. Ning, Z.-L., G.-F. Li, J. Wang, J.-F. Smith, H. Rasolonjatovo and M. Kang. 2013. Primulina huaijiensis (Gesneriaceae), a new species from Guangdong, China. Ann. Bot. Fenn. 50(1-2): 119–122.
   連結：
6. Posada, D. and K.-A. Crandall. 1998. ModelTest: testing the model of DNA substitution. Bioinformatics 14(9): 817–818.
   連結：
7. Ronquist, F., M. Teslenko, P.-V.-D. Mark, D.-L. Ayres, A. Darling, S. Höhna, B. Larget, L. Liu, M.-A. Suchard and J.-P. Huelsenbeck. 2012. MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice. Syst. Biol. 61(3): 539–542.
   連結：
8. Smissen, R.-D., I. Breitwieser and J.-M. Ward. 2004. Phylogenetic implications of trans-specific chloroplast DNA sequence polymorphism in New Zealand Gnaphalieae (Asteraceae). Plant Syst. Evol. 249(1-2): 37–53.
   連結：
9. Stamatakis, A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22(21): 2688–2690.
   連結：
10. Wang, Y.-Z., R.-B. Mao, Y. Liu, J.-M. Li, Y. Dong, Z.-Y. Li, and J.-F. Smith. 2011. Phylogenetic reconstruction of Chirita and allies (Gesneriaceae) with taxonomic treatments. J. Syst. Evol. 49(1): 50–64.
    連結：
11. Weber, A., D.-J. Middleton, A. Forrest, R. Kiew, C.-L. Lim, A.-R. Rafdah, S. Sontag, P. Triboun, Y.-G. Wei, T.-L. Yao and M. Möller. 2011. Molecular systematics and remodeling of Chirita and associated genera (Gesneriaceae). Taxon 60(3): 767–790.
    連結：
12. Xu, W.-B., H. Chang, J. Huang and K.-F. Chung. 2019. Molecular systematics of Chiritopsis-like Primulina (Gesneriaceae): one new species, one new name, two new combinations, and new synonyms. Bot. Stud. 60(1): 13–21.
    連結：
13. Wu, H.-T., S.-B. Zhou, W. Ma, O.-W. Wang. 2017. Primulina dichroantha (Gesneriaceae), a New Species from a Karst Cave from Guangxi, China. Ann. Bot. Fenn. 54(1-3): 95–98.
    連結：
14. Doyle, J.-J. and J.-L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
15. Fang, D. and D.-H. Qin. 2004. Wentsaiboea D. Fang et D.H. Qin, a new genes of Gesneriaceae from Guangxi, China. Acta Phytotax. Sin. 42: 533–536.
16. Hall, T.-A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41: 95–98.
17. Hance, H.-F. 1883. New Chinese Cyrtandreae. J. Bot. 21: 165–170. IPNI. 2020. The International Plant Names Index. [accessed 20 December 2020] https://www.ipni.org/
18. Wang, W.-T. 1986. Notulae de Gesneriaceis Sinensibus (VII). Guihaia 6: 1–15
19. Xu, W.-B., B. Pan, Y. Liu, C.-I. Peng and K.-F. Chung. 2012 Two new species, Primulina multifida and P. pseudomollifolia (Gesneriaceae), from karst caves in Guangxi, China. Bot. Stud. 53: 165–175.
20. Wen, F., Y.-G. Wei, L.-F. Fu, Z.-B. Xin, S. Li, Z.-J. Huang, Y.-Z. Ge, D.-C. Meng. 2020. The Checklist of Gesneriaceae in China. [accessed 20 December 2020] http://gccc.gxib.cn/cn/about-68.aspx