Morphological and molecular identification for Saxifraga yangshuoensis (sect. Irregulares, Saxifragaceae), a new species from Guangxi, China

10.6165/tai.2022.67.603

Hai-Ling CHEN；Wei-Bin XU；Wan-Yi ZHAO；Yan LIU

Chloroplast genome ； Irregulares ； Saxifraga damingshanensis ； Saxifraga shennongii

TAIWANIA

67卷4期（2022 / 12 / 01）

603 - 608

英文

Saxifraga yangshuoensis (Saxifragaceae), a new species from Yangshuo County in northeast Guangxi, China, is described and illustrated. It closely resembles S. damingshanensis and S. shennongii in stolons absent, leaves abaxially usually with spots, smallest petals base with yellow spotted, but differs from its leaves thickly papery, slightly revolute when dried, margin subentire, adaxially densely covered glandular hispid, abaxially densely covered with pale green spots, petiole base margin nearly glabrous and petals apex greenish. Combine morphological comparisons and phylogenetic analysis based on psbA-trnH and matK, confirmed that the new species differed from those similar species of S. sect. Irregulares.

1. Balfour, B. 1916. Saxifrages of the Diptera Section, with description. Transactions and proceedings of the Botanical Society of Edinburgh 27(1): 65–75.
   連結：
2. Curtis, W. 1774. XXXI. A catalogue of the fifty plants, from Chelsea Garden, presented to the Royal Society, by the Worshipful Company of Apothecaries, for the Year 1773, pursuant to the direction of Sir Hans Sloane, Bart. Med. Reg. et Soc. Reg. nuper Prœfes. Philosophical Transactions of the Royal Society of London 64(1): 302–309.
   連結：
3. Darriba, D., G.-L. Taboada, R. Doallo and D. Posada 2012. "jModelTest 2: more models, new heuristics and parallel computing". Nat. Methods. 9(8): 772.
   連結：
4. Deng, J., B.-T. Drew, E.-V. Mavrodiev, M.-A. Gitzendanner, P.-S. Soltis and E.-D. Soltis 2015. Phylogeny, divergence times, and historical biogeography of the angiosperm family Saxifragaceae. Mol. Phylogenet. Evol. 83: 86–98.
   連結：
5. Don, D. 1822. A monograph of the genus Saxifraga. Transactions of the Linnean Society of London 13(2): 341–452.
   連結：
6. Edgar, R.C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32(5): 1792–1797.
   連結：
7. Gornall, R.-J., H. Ohba and J.-T. Pan 2000. New taxa, names, and combinations in Saxifraga (Saxifragaceae) for the Flora of China. Novon. 10(4): 375–377.
   連結：
8. Ronquist, F., M. Teslenko, P. van der Mark , D.-L. Ayres, A. Darling, S. Höhna, B. Larget, L. Liu, M.-A. Suchard and J.-P. Huelsenbeck 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61(3): 539–542.
   連結：
9. Soltis, D.-E., L.-A. Johnson and C. Looney 1996. Discordance between ITS and chloroplast topologies in the Boykinia group (Saxifragaceae). Syst. Bot. 21(2): 169–185.
   連結：
10. Soltis, D.-E., R.-K. Kuzoff, M.-E. Mort, M. Zanis, M. Fishbein, L. Hufford, J. Koontz and M.-K. Arroyo 2001. Elucidating deeplevel phylogenetic relationships in Saxifragaceae using sequences for six chloroplastic and nuclear DNA regions. Ann. Mo Bot. Gard. 88 (4): 669–693.
    連結：
11. Soltis, D.-E., M.-M. Mort, M. Latvis, E.-V. Mavrodiev, B.-C. O’Meara, P.-S. Soltis, J.-G. Burleigh and R. Rubio De Casas 2013. Phylogenetic relationships and character evolution analysis of Saxifragales using a supermatrix approach. Am. J. Bot. 100(5): 916–929.
    連結：
12. Stamatakis, A., P. Hoover and J. Rougemont 2008. A rapid bootstrap algorithm for the RAxML web-servers. Syst. Biol. 57(5): 758–771.
    連結：
13. Tkach, N., M. Röser, G. Miehe, A.-N. Muellner-Riehl, J. Ebersbach, A. Favre and M.-H. Hoffmann 2015. Molecular phylogenetics, morphology and a revised classification of the complex genus Saxifraga (Saxifragaceae). Taxon. 64(6): 1159–1187.
    連結：
14. Wang, G.-F., Y.-S. Ye and F.-W. Xing 2008. Saxifraga daqiaoensis (Saxifragaceae), a new species from Guangdong, China. Ann. Bot. Fenn. 45(3): 237–239.
    連結：
15. Xiang, C.-L., M.-A. Gitzendanner, D.-E. Soltis, H. Peng and L.-G. Lei 2012. Phylogenetic placement of the enigmatic and critically endangered genus Saniculiphyllum (Saxifragaceae) inferred from combined analysis of plastid and nuclear DNA sequences. Mol. Phylogenet. Evol. 64(2): 357–367.
    連結：
16. Zhang, D., F.-L. Gao, I. Jakovlić, H. Zou, J. Zhang, W.-X. Li and G.-T. Wang 2020a. PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Mol. Ecol. Resour. 20(1): 348–355.
    連結：
17. Zhang, J.-J., W.-Y. Zhao, K.-K. Meng, W.-B. Liao, L. Wang and S.-F. Chen 2019. Saxifraga shennongii, a new species of Saxifragaceae from Hunan Province, China. Phytotaxa. 418(1): 79–88.
    連結：
18. Zhang, M.-H., X.-S. Zhang, Z.-L. Nie, D.-G. Zhang and Y. Meng 2017. Saxifraga kegangii (Saxifragaceae), a new species from Hunan Province of central China. Phytotaxa. 309(2): 159–165.
    連結：
19. Zhang, M.-H., C.-Y. Wang, C. Zhang, D.-G. Zhang, K.-G. Li, Z.-L. Nie and Y. Meng 2020. Phylogenetic relationships and biogeographic history of the unique Saxifraga sect. Irregulares (Saxifragaceae) from eastern Asia. J. Syst. Evol. 58(6): 958–971.
    連結：
20. Zhang, X.-J., Q.-S. Fu, J.-T. Chen, L.-J. Li, P.-R. Luo, J.-Y. Peng, X.-H. Huang, H. Sun and T. Deng 2021. Saxifraga viridiflora (Saxifragaceae), an unusual new species from Guangxi, China. PhytoKeys. 184: 19–26.
    連結：
21. Zhang, X.-J., T.-H. Kuang, J.-T. Chen, L.-J. Li, J.-Y. Peng, K.-L. Guan, X.-H. Huang, H.-C. Wang and T. Deng 2022. Saxifraga sunhangiana (Saxifragaceae), a new species from Gansu, China. Taiwania. 67(2): 195–200.
    連結：
22. Zhang, X.-J., Z.-C. Liu, K.-K. Meng, Q.-L. Ding, L. Wang and W.-B. Liao 2018. Saxifraga luoxiaoensis (Saxifragaceae), a new species from Hunnan and Jaingxi, China. Phytotaxa. 350(3): 291–296.
    連結：
23. Zhao, W.-Y., K.-K. Meng, Q. Fan, J.-H. Jin and W.-B. Liao 2019. Saxifraga damingshanensis (S. sect. Irregulares, Saxifragaceae), a new species from Guangxi, China. Phytokeys. 133: 95–103.
    連結：
24. Doyle, J.-J. and J.-L. Doyle 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bulletin. 19(1): 11–15.
25. Engler, A. and E. Irmscher 1913. Diagnosen neuer arten. Notizblatt des Königlichen Botanischen Gartens und Museums zu Berlin (-Dahlem). 6: 35–37.
26. Hall, T.-A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analyses program for Windows 95/98/NT. Nucleic Acids Symposium Series. 41: 95–98.
27. Haworth, A.-H. 1803. Miscellanea naturalia: sive dissertationes variæ ad historiam naturalem spectantes. Typis J. Taylor, Londini, 155 pp.
28. IUCN 2012. IUCN Red List Categories and Criteria: Version 3.1. Second edition. IUCN, Gland, Switzerland and Cambridge, iv + 32 pp.
29. Linnaeus, C. 1753. Species plantarum. Laurentius Salvius, Stockholm, pp. 398–405.
30. Pan, J.-T. 1991. A Study on the Genus Saxifraga L. from China. Acta Phytotaxon. Sin. 29: 1–24.
31. Tate, J.-A. and B.-B. Simpson 2003. Paraphyly of Tarasa (Malvaceae) and diverse origins of the polyploidy species. Syst. Bot. 28(4): 723–737.