A new natural hybrid Goodyera ×kazumae (Orchidaceae) from Tomakomai City, Hokkaido Prefecture, Japan

10.6165/tai.2024.69.309

Kenji SUETSUGU；Shun K. HIROTA；Yoshihisa SUYAMA

DNA barcoding ； Goodyera henryi ； Goodyera schlechtendaliana ； hybridization ； MIG-seq

TAIWANIA

69卷3期（2024 / 09 / 01）

309 - 316+s1-2

英文

Several individuals of an unidentified Goodyera taxon were discovered in Tomakomai City, Hokkaido Prefecture, Japan. Detailed morphological analysis suggests that this taxon is a natural hybrid between Goodyera schlechtendaliana and Goodyera henryi, based on characteristics such as leaf margins, texture, venation patterns, rachis length, hair morphology on the ovary, and the shapes of the lip apex and rostellum. Supporting this, molecular data from genome-wide markers using next-generation sequencing (MIG-seq data) indicate that G. ×kazumae is a hybrid between G. schlechtendaliana and G. henryi. Neighbor-Net phylogenetic analysis shows G. ×kazumae occupies an intermediate position between the putative parent species. STRUCTURE analysis further confirms that G. ×kazumae comprises genetic components from both species. Therefore, we conclude that this taxon is a natural hybrid based on comprehensive morphological and molecular evidence.

1. Earl, D.A., vonHoldt, B.M. 2012 STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv. Genet. Resour. 4(2): 359–361.
   連結：
2. Evanno, G., Regnaut, S., Goudet, J. 2005 Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol. 14(8): 2611–2620.
   連結：
3. Guan, Q.X., Chen, G.Z., Li, M.H., Chen, S.P. 2014 Goodyera malipoensis (Cranichideae, Orchidaceae), a new species from China: Evidence from morphological and molecular analyses. Phytotaxa 186(1): 51–60.
   連結：
4. Hirano, T., Saito, T., Tsunamoto, Y., Koseki, J., Prozorova, L., Do, V.T., Matsuoka, K., Nakai, K., Suyama, Y., Chiba, S. 2019 Role of ancient lakes in genetic and phenotypic diversification of freshwater snails. Mol. Ecol. 28(23): 5032–5051.
   連結：
5. Hu, C., Tian, H., Li, H., Hu, A., Xing, F., Bhattacharjee, A., Hsu, T., Kumar, P., Chung, S. 2016 Phylogenetic analysis of a ‘jewel orchid’ genus Goodyera (Orchidaceae) based on DNA sequence data from nuclear and plastid regions. Plos One 11(2): e0150366.
   連結：
6. Huson, D.H., Bryant, D. 2006 Application of phylogenetic networks in evolutionary studies. Mol. Biol. Evol. 23(2): 254–267.
   連結：
7. Kallunki, J.A. 1976 Population studies in Goodyera (Orchidaceae) with emphasis on the hybrid origin of G. tesselata. Brittonia 28(1): 53–75.
   連結：
8. Kallunki, J.A. 1981 Reproductive biology of mixed-species populations of Goodyera (Orchidaceae) in northern Michigan. Brittonia 33(2): 137–155.
   連結：
9. Kopelman, N.M., Mayzel, J., Jakobsson, M., Rosenberg, N.A., Mayrose, I. 2015 CLUMPAK: a program for identifying clustering modes and packaging population structure inferences across K. Mol. Ecol. Resour. 15(5): 1179–1191.
   連結：
10. Lee, C.S., Kim, S.-C., Yeau, S.H., Lee, N.S. 2012 Nuclear and cpDNA sequences demonstrate spontaneous hybridization between Goodyera schlechtendaliana Rchb. f. and G. velutina Maxim. (Orchidaceae) in Jeju Island, Korea. Syst. Bot. 37(2): 356–364.
    連結：
11. Lee, C.S., Yeau, S.H., Lee, K.S., Lee, N.S. 2010 A new taxon of Goodyera (Orchidaceae): G. ×tamnaensis. Korean J. Pl. Taxon. 40(4): 251–254.
    連結：
12. Nakahama, N., Suetsugu, K., Ito, A., Hino, M., Yukawa, T., Isagi, Y. 2019 Natural hybridization patterns between widespread Calanthe discolor (Orchidaceae) and insular Calanthe izu-insularis on the oceanic Izu Islands. Bot. J. Linn. 190(4): 436–449.
    連結：
13. Oh, S.-H., Suh, H.-J., Seo, S.-W., Chung, K.-S., Yukawa, T. 2022 A new species of Goodyera (Orchidaceae: Orchidoideae) from Korea and Japan. J. Pl. Biol. 65(5): 357–363.
    連結：
14. Pritchard, J.K., Stephens, M., Donnelly, P. 2000. Inference of population structure using multilocus genotype data. Genetics 155(2): 945–959.
    連結：
15. Rochette, N.C., Rivera-Colón, A.G., Catchen, J.M. 2019 Stacks 2: Analytical methods for paired-end sequencing improve RADseq-based population genomics. Mol. Ecol. 28(21): 4737–4754.
    連結：
16. So, J.-H., Lee, N.-S. 2017 The origin of new natural hybrid, Goodyera ×maximo-velutina (Orchidaceae) from Jeju Island, Korea. Phytotaxa 317(1): 61–68.
    連結：
17. Suetsugu, K., Hirota, S.K., Nakato, N., Suyama, Y., Serizawa, S. 2022 Morphological, ecological, and molecular phylogenetic approaches reveal species boundaries and evolutionary history of Goodyera crassifolia (Orchidaceae, Orchidoideae) and its closely related taxa. PhytoKeys 212: 111–134.
    連結：
18. Suetsugu, K., Hirota, S.K., Shitara, T., Ishida, K., Nakato, N., Hayakawa, H., Suyama, Y. 2024 The absence of bumblebees on an oceanic island blurs the species boundary of two closely related orchids. New Phytol. 241: 1321–1333.
    連結：
19. Suetsugu, K., Hirota, S.K., Suyama, Y. 2021a A new natural hybrid, Goodyera ×tanakae (Orchidaceae) from Japan with a discussion on the taxonomic identities of G. foliosa, G. sonoharae, G. velutina, G. ×maximo-velutina and G. henryi, based on morphological and molecular data. Taiwania 66(3): 277‒286.
    連結：
20. Suetsugu, K., Hirota, S.K., Suyama, Y. 2021b First record of Goodyera × tamnaensis (Orchidaceae) from Boso Peninsula, Chiba Prefecture, Japan, based on morphological and molecular data. Taiwania 66(1): 113–120.
    連結：
21. Suetsugu, K., Shitara, T., Nakato, N., Ishida, K., Hayakawa, H. 2019 First record of Goodyera ×maximo-velutina (Orchidaceae) from Kozu Island, Japan. Taiwania 64(4): 347–352.
    連結：
22. Suyama, Y., Hirota, S.K., Matsuo, A., Tsunamoto, Y., Mitsuyuki, C., Shimura, A., Okano, K. 2022 Complementary combination of multiplex high-throughput DNA sequencing for molecular phylogeny. Ecol. Res. 37(1): 171–181.
    連結：
23. Tamaki, I., Yoichi, W., Matsuki, Y., Suyama, Y., Mizuno, M. 2017 Inconsistency between morphological traits and ancestry of individuals in the hybrid zone between two Rhododendron japonoheptamerum varieties revealed by a genotyping-by-sequencing approach. Tree Genet. Genomes 13(1): 1–10.
    連結：
24. Yoichi, W., Kawamata, I., Matsuki, Y., Suyama, Y., Uehara, K., Ito, M. 2018 Phylogeographic analysis suggests two origins for the riparian azalea Rhododendron indicum (L.) Sweet. Heredity 121(6): 594–604.
    連結：
25. Govaerts, R., Bernet, P., Kratochvil, K., Gerlach, G., Carr, G., Alrich, P., Pridgeon, A., Pfahl, J., Campacci, M., Holland Baptista D., Tigges, H., Shaw, J., Cribb, P., George, A.S., Kreuz, K., Wood, J. 2022 World Checklist of Orchidaceae. Royal Botanic Gardens, Kew, UK. http://wcsp.science.kew.org/
26. Pridgeon, A.M., Cribb, P.J., Chase, M.W., Rasmussen, F.N. 2003 Genera Orchidacearum 3. Orchidoideae (part 2), Vanilloideae. Oxford: Oxford University Press.
27. Suetsugu, K., Hayakawa, H. 2019 A new variety of Goodyera schlechtendaliana (Orchidaceae) from Yakushima and Okinawa, Japan. Acta Phytotax. Geobot. 70: 49–55.
28. Thiers, B. 2023 Index Herbariorum: A Global Directory of Public Herbaria and Associated Staff. New York Botanical Garden’s Virtual Herbarium.