Soil nematode fauna in rice paddy fields from Taiwan based on morphology and DNA barcoding

10.6165/tai.2024.69.420

Ming-Chung CHIU；Tang-Wei LIN；Zhao-Hui LIN；Li-Yen LEE；Po-Wei HSU；Hsuan-Wien CHEN

free-living nematode ； functional group ； molecular identification ； morphological description ； rice paddy field

TAIWANIA

69卷3期（2024 / 09 / 01）

420 - 434

英文

Nematodes adapt to variety of environments. Dynamics of a nematode community are highly associated with the soil condition, which can serve as an indicator for monitoring the impact of human activity on the soil ecosystem. However, a systematic study on nematode fauna is lacking in many regions across Taiwan. In this study, we provide morphological and molecular identification for the soil nematodes collected during the cultivation process of the first crop rice in Chiayi, Taiwan. Within the three-year survey, 26 morphospecies of 18 genera were identified in the soil from the rice paddy fields. Each morphospeices was initially identified based on morphology, and 11 were further examined using 18S rDNA sequences. Among these, nine predominant morphospeices represented over 90% of the abundance, while juveniles of the plant pathogenetic nematode, Meloidogyne, were the most abundant one. Bacterivore was the most diverse and abundant functional group in the nematode community. Eight morphospecies were supported by the molecular identification at the genus level, while three require further consideration due to inconsistencies between morphological and molecular analyses.

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