Establishing a Cell Line from Apolemichthys trimaculatus Fins

藍嘴神仙（Apolemichthys trimaculatus）鰭細胞株之建立

10.29822/JFST.201306_40(2).0003

林學廉(Shiue-Lian Lin)；陳秀男(Shiu-Nan Chen)

海水觀賞魚 ； 細胞株 ； 病毒 ； 鰭 ； marine ornamental fish ； cell line ； virus ； fin

臺灣水產學會刊

40卷2期（2013 / 06 / 01）

107 - 116

英文

在這項研究中，我們建立了一個新的細胞株，它是從高經濟價值的海水觀賞魚－藍嘴神仙（Apolemichthys trimaculatus）的鰭組織所建立，新的細胞株命名為TSAF。TSAF細胞株的生長和繼代培養，可使用Leibovitz的L15加上10%胎牛血清，在28℃溫度下長成纖維細胞形態的細胞株，並進行了100代的繼代培養。TSAF細胞株的最適生長溫度在28℃至32℃，具有38個染色體數目。TSAF細胞株可容易地培養出台灣鰻魚皰疹病毒（EHVF），歐洲鰻病毒（EVE），傳染性胰臟壞死病毒（IPNV）的血清型AB，SP，VR299，文蛤呼腸孤病毒（HCRV）等。這種被病毒廣受性感染的能力，明白指出TSAF細胞株是有助於的研究魚類病毒疾病的價值，特別是可應用於海水觀賞魚的物種。

In this study, we established a new cell line derived from a marine ornamental fish, Apolemichthys trimaculatus, which is considered a species of high economic value. The new cell line, designated as TSAF, was established using the fin tissue of a marine angelfish. The cell line was grown and subcultured in Leibovitz L15 with 10% fetal bovine serum (FBS) and incubated at 28℃. The TSAF, a fibroblastic morphological cell line, was subcultured more than 100 times after its primary culture. The optimal growth temperature for TSAF ranged from 28℃ to 322℃, and its modal chromosomal number was measured at 38. The cells were susceptible to eel herpesvirus from Formosa (EHVF), eel virus European (EVE), infectious pancreatic necrosis virus serotype AB, SP, VR299 (IPNV), and hard clam reovirus (HCRV). This wide-range viral susceptibility suggests that TSAF should be valuable for studying fish viruses, especially those of ornamental fish.