蝴蝶蘭屬原種之花梗芽在NDM培養基中培養之表現

Characteristics of Flower Stalk Bud of Phalaenopsis Species Proliferated on NDM Medium

10.6964/JCSHS.200512.0377

漥田浩一(Koichi Kubota)；藤木俊也(Toshiya Fujiki)；雨宫圭一(Keiichi Amemiya)；米田和夫(Kazuo Yoneda)

花梗芽培養 ； 營養系增生 ； 蝴蝶蘭屬 ； 種 ； NDM ； flower stalk bud culture ； NDM ； Phalaenopsls ； proliferation ； species

中國園藝

51卷4期（2005 / 12 / 01）

377 - 379

繁體中文

以蝴蝶蘭屬原種15種之21品種作爲材料，在添加NAA0.1mg/L，BA1mg/L，蔗糖10g/L，pH5.4的NDM培養基中，進行花梗芽培養，6原種，12品種中，有25-83%的培植體形成PLB，以Phal. equestris 'Hanajima'的PLB形成率較高。另外，迄今爲止關於花梗芽的培養尚無研究報告的種群，如Phal. fasciata形成了25%的PLB，除此以外，Phal. Fasciata ('JS﹟1'×'Dr. Kou')，Phal. inscriptogenesis' Phal. lamelegra, Phal. lueddemannianna var. purpurea, Phal. mannii和Phal. schilleriana等6種則完全沒有形成PLB。

Clonal method using PUB was established by flower stalk bud culture in Phalaenopsis species. Flower stalk buds were cultured on New Dogashima Medium (NDM) supplemented with NAA and BA Those buds were incubated at 25℃ under 4000 lux After 2 month 25 to 83% of the cultured buds produced PUB in 12 of 21 cultivars. As the result, it seemed that flower stalk bud culture was an effective method for PLB formation in some cultivars.