Low Intensity Ultrasoud Treatment Accelerates Bone Repair in a Rat Femur Fracture Model

以低強度超音波治療加速老鼠股骨骨折癒合

王世杰(S. J. Wang)；D. G. Lewallen；M. E. Bolander；J. F. Greenleaf；Edmund Y. S. Chao

Fracture repair ； Ultrasound therapy ； Mechanical strength ； Endochondral ossification

Journal of Orthopaedic Surgery Taiwan

10卷1期（1993 / 03 / 01）

13 - 24

英文

Bilateral closed femoral shaft fractures were made in 22 male Long-Evans rats by a closed method. In 16 animals ultrasound was applied to one limb for 15 minutes daily 10 times within the first 14 postoperative days. The treated limbs received a 200 us burst of 1.5 MHz or 0.5 MHz sine waves repeated at 1.0 MHz at a spatial average temporal averaged intensity of 30 W/cm2. Contralateral limbs served as non-treated controls. Siz remaining animals with fractures and 6 additional aninals without fractures received sham ultrasound treatment to control for the effects of anasethesia and handling. Fracture repair was evaluated by radiography, mechanical testing in torsion, and histology on postoperative day 21. Five of 16 ultrasound-treated fractures and none of the 28 controls showed obliteration of the fracture gap on radiographs. The average maximum torque of fractures treated with either signal was 22% greater than contralateral controls (P＜0.05). The stiffness of treated fractures was greater than control fractures, but the difference was significant only in animals treated with the 1.5 MHz signal (P＜0.02). Histologic evaluation suggested more advanced endochondral ossification in 1- of the 16 ultrasound-treated fractures when compared to contralateral controls. Sham treatment did not affect repair in the control group. These results indicate that low-intensity pulsed ultrasound can accelerate early fracture repair in this highly controlled model.

Bilateral closed femoral shaft fractures were made in 22 male Long-Evans rats by a closed method. In 16 animals ultrasound was applied to one limb for 15 minutes daily 10 times within the first 14 postoperative days. The treated limbs received a 200 us burst of 1.5 MHz or 0.5 MHz sine waves repeated at 1.0 MHz at a spatial average temporal averaged intensity of 30 W/cm2. Contralateral limbs served as non-treated controls. Siz remaining animals with fractures and 6 additional aninals without fractures received sham ultrasound treatment to control for the effects of anasethesia and handling. Fracture repair was evaluated by radiography, mechanical testing in torsion, and histology on postoperative day 21. Five of 16 ultrasound-treated fractures and none of the 28 controls showed obliteration of the fracture gap on radiographs. The average maximum torque of fractures treated with either signal was 22% greater than contralateral controls (P＜0.05). The stiffness of treated fractures was greater than control fractures, but the difference was significant only in animals treated with the 1.5 MHz signal (P＜0.02). Histologic evaluation suggested more advanced endochondral ossification in 1- of the 16 ultrasound-treated fractures when compared to contralateral controls. Sham treatment did not affect repair in the control group. These results indicate that low-intensity pulsed ultrasound can accelerate early fracture repair in this highly controlled model.

1. 陳彥安(2016)。研發人工皮膚仿體應用於電刺激治療。國立臺灣大學機械工程學系學位論文。2016。1-92。