In Vitro Osteoblastic Differentiation of Rabbit Mesenchymal Stem Cells in a Poly (Lactide-Co-Glycolide)/Interpore/Collagen Composite System

使用PLGA/Interpore/第一型膠原作為兔子幹細胞載體在體外的骨分化測試

黃炤文(Jau-Wen Huang)；林松樞(Song-Shu Lin)；陳文哲(Wen-Jer Chen)；劉士榮(Shih-Jung Liu)；牛自健(Chi-Chien Niu)；陳力輝(Lih-Huei Chen)；袁立仁(Li-Jen Yuan)

mesenchymal stem cell ； bone substitute ； PLGA ； collagen ； interpore

Journal of Orthopaedic Surgery Taiwan

27卷2期（2010 / 06 / 01）

71 - 79

英文

Purpose: Bone grafting is widely used in clinical orthopedic surgery. In order to avoid the complications associated with autogenous bone grafting, many kinds of bone substitutes have been developed. We developed an MSC carrier comprising a hot compression-molded polylactide/polyglycolide (PLGA), a porous calcium phosphate product that is used clinically and commercially (Interpore; Pro-Osteon 500R, Irvine, California) and a type I collagen composite for use in bone grafting. The purpose of this study was to evaluate the osteoblastic differentiation ability of rabbit MSC s loaded on this PLGA /Interpore/type I collagen carrier system in vitro. Materials & Methods: MSC s aspirated from rabbit bone marrow were cultured in induction medium and loaded onto the PLGA /Interpore/type I collagen carrier system. After culture for 7 and 14 days, the osteoblastic differentiation ability was tested using histological analysis and reverse transcription polymerase chain reaction (RT-PCR ) and by measuring the calcium and alkaline phosphatase levels. Results: After culture in the induction medium for 7 and 14 days, both the calcium and alkaline phosphatase levels were significantly higher than those in the control group. RT -PCR revealed mRNA expression of osteopontin, core binding factor alpha 1 (Cbfa1), and type I collagen. Optical micrographs of the MSC-loaded PLGA /Interpore/type I collagen carrier surface were obtained. After culture in osteogenic medium for 14 days, the carrier surface was almost entirely covered by cells and a dense collagenous extracellular matrix. Conclusions: The results of the study suggest that the biodegradable PLGA / Interpore/type I collagen MSC carrier system possesses potential osteoblastic differentiation ability.