高尾山蟲草無性世代（Paecilomyces tenuipes）發酵產物在抗氧化及促進吞噬能力之初步研究

A Preliminary Study on the Anti-oxidant and Phagocytosis from the Fermentation of Anamorph Paecilomyces tenuipe

周坤儒(Kun-Ru Chou)；陳啟楨(Chee-Jen Chen)；黃榆婷(Yu-Ting Huang)

高尾山蟲草 ； 抗氧化 ； 吞噬能力 ； Paecilomycestenuipes ； anti-oxidant ； Phagocytosis

Journal of Biomedical & Laboratory Sciences

25卷3期（2013 / 09 / 01）

91 - 97

繁體中文

蟲草屬真菌（Cordyceps spp.）是一群藥用真菌，具有廣泛的生物及藥理活性，在許多藥理學研究中可發現，蟲草屬真菌具有抗腫瘤、免疫調節、抗氧化及保肝等作用。本篇研究題材為高尾山蟲草無性世代（Paecilomyces tenuipes），探討其發酵產物在抗氧化及提升老鼠巨噬細胞（RAW 264.7）吞噬能力。實驗結果發現，P. tenuipes發酵後產物具提升巨噬細胞吞噬能力，以菌絲體熱水萃取物（1mg/mL）為最佳。熱水萃取菌絲體具有刺激老鼠巨噬細胞產生一氧化氮（NO）能力，且具有劑量遞增性效果。在抗氧化能力方面，發酵液具有不錯清除DPPH能力。再進一步使用流式細胞儀來偵測CHO-K1細胞氧化程度。結果顯示，高尾山蟲草發酵產物可降低H2O2對CHO-K1細胞內產生的氧化傷害。希望這些發現可對高尾山蟲草研究有所助益。

The genus Cordyceps is a group of precious medicinal mushrooms which possess a wide of biological and pharmacological activities. The medicinal effects, such as anti-tumor, immunomodulation, antioxidant activity and hepatoprotective effect, had been studied in past years. This study on the anti-oxidant and Phagocytosis from the fermentation of anamorph Paecilomyces tenuipe. The hot crude extract of mycelia could significantly enhance phagocytosis activity on RAW 264.7 (p＜0.05). Meanwhile, enhancing nitric oxide activities on macrophage cellsin adose-dependent evaluated by Griess method. In the anti-oxidized aspect, the fermentation filtrate had also shown a great activity by scavenging the DPPH. The antioxidant activity was assayed by Flow cytometry, and the result showed that the fermentation filtrate had a great antioxidant activity. These results suggest that Paecilomyces tenuipe indeed possessed some biology activities. Hopefully, these findings are useful to further studies on bioactivity of Paecilomyces tenuipe.