自配酒精酵素試劑定量酒精方法之評估

Evaluation of Enzymatic Assay for Ethanol Quantitation

10.6440/TZUCMJ.199409.0171

林碧芬(Bih-Fen Lin)；賴滄海(Ahai C. Lua)

酒精 ； 自動化 ； 酵孝法 ； ethanol ； automation ； enzymatic assay

慈濟醫學雜誌

6卷3期（1994 / 09 / 01）

171 - 178

繁體中文

The reagent for the enzymatic assay of ethanol was prepared and adapted to automated clinical chemistry analyzer (Hitachi 747) and 96-well microplate with automated ELISA Reader. These two methods were compared with the Siama commercial kits. The precision of these three methods is very high, and the percentages of coefficient of coefficient of variance (CV%) are all less than 4% except at the low ethanol concentration by the microplate method. There is no matrix effect by the Hiachi 747 method and the Sigma Kits, and the matrix effect of the microplate method could be eliminated by using a normal sample of the same matrix as a blank. As reported by others, n-butanol and isopropanol interfere with the assay, but the addition of glucose (5 mg/ml) has no effect. The three methods correlate well with each other (r2＞0.96). In conclusion, these three methods could be used exchangably, and the concentration of the measured ethanol is not affected,. (Tzu-Chi Med J 1994; 6: 171-178)

The reagent for the enzymatic assay of ethanol was prepared and adapted to automated clinical chemistry analyzer (Hitachi 747) and 96-well microplate with automated ELISA Reader. These two methods were compared with the Siama commercial kits. The precision of these three methods is very high, and the percentages of coefficient of coefficient of variance (CV%) are all less than 4% except at the low ethanol concentration by the microplate method. There is no matrix effect by the Hiachi 747 method and the Sigma Kits, and the matrix effect of the microplate method could be eliminated by using a normal sample of the same matrix as a blank. As reported by others, n-butanol and isopropanol interfere with the assay, but the addition of glucose (5 mg/ml) has no effect. The three methods correlate well with each other (r2＞0.96). In conclusion, these three methods could be used exchangably, and the concentration of the measured ethanol is not affected,. (Tzu-Chi Med J 1994; 6: 171-178)