Absence of CCR5-Δ32 Mutation in Healthy and HIV-1-Infected Aborigines in Eastern Taiwan

台灣東部健康及HIV-1感染原住民CCR5-Δ32突變基因之現況

10.6440/TZUCMJ.200212.0353

程兆明(Jaw-Ming Cherng)；王志鴻(Ji-Hung Wang)；許文林(Wen-Lin Hsu)；楊國梁(Kuo-Liang Yang)；林孝義(Hsiao-Yi Lin)；王崇任(Chrong-Reen Wang)；林繼謨(Chi-Mo Lin)；王立信(Lih-Shinn Wang)

愛滋病毒，CCR5，削除性突變，種族差異 ； Human immunodeficiency virus type 1 ； CCR ； deletion mutation ； ethnic variation

慈濟醫學雜誌

14卷6期（2002 / 12 / 01）

353 - 357

英文

目的：愛滋病毒HIV-1需要coreceptor CCR5以進入宿主細胞。CCR5基因之32核甘酸的同型削除性突變(CCR5-Δ32)會導致分子嚴重之截斷以及對HIV-1感染有完全之保護作用。白種人常有此種削除性突變。但是至今香港及台灣未發現有此種突變。因為現今的資料大部份來自非原住民而且部份原住民被認為是來自歐洲以及其他東南亞地方，並非全然來自中國大陸。為對台灣東部之現況有更深入之瞭解，本研究為探討台灣東部健康原住民及HIV-1感染患者的CCR5-Δ32突變基因之現況。材料與方法：利用PCR方法，對1,433位正常人以及11位HIV-1感染原住民進行CCR5基因突變的檢測。結果：發現所有結果皆為野生型之CCR5，無一為CCR5-Δ32。結論：雖然部份台灣原住民被認為來自歐洲以及其他東南亞地方，並非全然來自中國大陸，但是台灣原住民與非原住民則有相似之CCR5基因。

Objective: Human immunodeficiency virus type 1 requires coreceptor CCR5 to enter into host cells. Homozygous deletion of 32 nucleotides (CCR5-Δ32) of CCR5 results in a severely truncated molecule and near-complete protection against HIV-1 infection. This deletion mutation is commonly found in Caucasians. However, no such a mutation has yet been found in Hong Kong and Taiwan. Furthermore, most current data in Taiwan were collected from non-aborigines. Therefore, we investigated whether CCR5-Δ32 mutation occurs in aborigines in eastern Taiwan. Materials and Methods: Genomic DNA was extracted from healthy aborigines ( n=1433) and HIV-1 infected aboriginal patients ( n=11) in eastern Taiwan. Genotyping was performed using polymerase chain reaction (PCR) amplification of a portion of the CCR5 gene and analyzed with agarose gel electrophoresis. Results: All samples contained the wild-type CCR5 gene structure and no single deleted form of the CCR5 gene was found. Conclusion: Although some Taiwan aborigines are believed to have European and other ancestors as well as mainland Chinese ancestors, Taiwan aborigines have a CCR5 gene construction similar to non-aboriginal Taiwanese.