以ISSR DNA標誌用於苦瓜遺傳變異及品種鑑定之研究

Analysis of the Genetic Diversity and Variety Identification of Bitter Gourd (Momordica charantia L.) by ISSR Markers

10.6957/RBHDAIS.201011.0021

黃佳興(Chia-Hsing Huang)；王啟正(Chii-Jeng Wang)；全中和(Jong-Ho Chyuan)

苦瓜 ； 品種鑑定 ； 簡單序列重複區間 ； bitter gourd ； variety identification ； ISSR (Inter Simple Sequence Repeat)

花蓮區農業改良場研究彙報

28期（2010 / 11 / 01）

21 - 33

繁體中文

為了進行苦瓜品種的分子鑑定，本研究使用27個ISSR分子標誌對38個苦瓜品種（系）進行遺傳分析，共產生247個條帶，其中115個具多型性，平均每個引子提供4.25個多型性條帶，各引子產生多型性條帶數目從0至9皆有，分子量範圍從150至2700 bp，品種（系）間最低和最高的遺傳相似性分別是0.508和0.969。群集分析結果顯示野生種和經濟栽培品種的遺傳相似性最低，而經濟栽培種間的相似度最高。另外，針對本研究收集的18個台灣栽培種及東南亞栽培種之後代進行品種鑑定發現只需6個ISSR引子即可有效的區分這18個品種（系），表示ISSR分子標誌可有效且方便的運用在苦瓜品種鑑定上，在苦瓜的遺傳相似性研究中發現台灣的野生種苦瓜比本國及東南亞的栽培種在親遺傳相似度上較小，這可作為苦瓜育種工作上的參考。

For identification and genetic diversity of bitter gourd, 27 ISSR markers were chosen to perform a genetic analysis of 38 diverse bitter gourd accessions. The 247 amplicons yielded from ISSR-PCR analysis and 115 polymorphic were included. Each primer produced 4.25 polymorphic bands in average. Numbers of polymorphic amplicon were 0 to 9 and each size of products ranged from 150 to 2700 bp. The lowest and highest genetic similarity coefficients were 0.508 and 0.938, respectively. The phylogenetic analysis indicating that similarity between wild varieties and general cultivars is relative far, but the similarity among general cultivars is very near. The 18 accessions including Taiwan cultivars and descendants of South-East Asia cultivars were chosen for variety identification study. The results show that discrimination for the 18 accessions just needs 6 ISSR primers and these primers are effective and convenient for bitter gourd identification. Genetic diversity of wild varieties from Taiwan is higher than the other accessions. These data will be a good reference for bitter gourd breeders.