Chemical Identification and Bioactivity of Rat (Rattus Rattus) Urinary Compounds

家鼠（Rattus rattus）尿液成分的鑑定及其生物活性的分析

Ramasamy Selvaraj；Govindaraju Archunan

尿液 ； 費洛蒙 ； 化學特性 ； 社會行為 ； 鼠 ； Urine ； Pheromone ； Chemical characterization ； Social behavior ； Rat

Zoological Studies

41卷2期（2002 / 04 / 01）

127 - 135

英文

本研究利用氣相層析質譜儀分析家鼠的尿液，發現雄鼠的尿液中含有2-(octylthio) ethanol、1,3,5-trizone-2,4-diamine和1-chlorodecane。雌鼠在發情期時的尿液中則含有Hydroperoxide、1-intropentane和4-azidoheptane。利用Y型迷宮來檢測家鼠對氣味的喜好程度，結果顯示這些成分的氣味對異性與同性的家鼠都具有吸引力。雄鼠的ethanol,2-(octylthio)和1-chlorodecane吸引雌鼠，1,3,5-trizone-2,4-diamine則吸引雄鼠。雌鼠尿液的Hydroperoxide和4-azidoheptane顯示出其最大的作用在於吸引異性老鼠，1-nitropentane則對兩性都有吸引作用，但對吸引異性比較有效。本研究的結果顯示老鼠尿液中這些被鑑定的成分具有性別專一性，且含有揮發性物質， 其生物活性亦各不相同。

House rat urine was analyzed to identify the chemical compounds by gas chromatography-linked mass spectrometry (GC-MS). Male rat urine contained ethanol,2-(octylthio) (I), 1,3,5 triazone-2,4-diamine (II), and 1-chlorodecane (III). Similarly, female urine (during estrus) had the 3 compounds hydroperoxide (IV), 1-nitropentane (V), and 4-azidoheptane (VI). The bioactivity of these identified compounds were assayed using a Y-maze apparatus. Odor preference test revealed that the identified compounds show opposite-sex as well as same-sex attraction. Male urinary compounds such as ethanol,2-(octylthio) (I) and 1-chlorodecane (III) exhibited an attraction to the opposite sex, whereas the compound 1,3,5 triazone-2,4-diamine (II) was shown to attract the same sex. However, the compounds hydroperoxide and 4-azidoheptane were found to show maximum response in the attraction of the opposite sex. By contrast, the compound 1-nitropentane attracted both sexes, with a maximum response to the opposite sex. The results indicate that the compounds identified in the male urine are sex specific. The present investigations also show that rat urine contains a mixture of volatile compounds whose bioactivities differ from compound to compound.