Distribution of Precerebellar Cholecystokininergic Neurons in the Perihypoglossal Nuclei of the Gerbil

沙鼠舌下神經周核內投射至小腦之膽囊激素性的神經元分布

李世雄(Shih-Hsiung Lee)；曾啟育(Chi-Yu Tseng)；溫振源(Chen-Yuan Wen)；謝正勇(Jeng-Yung Shieh)

膽囊激素 ； 雙重標示 ； 舌下神經周核投射神經元 ； 神經追蹤劑 ； 囓齒類 ； Cholecystokinin ； Double-labelling ； Perihypoglossal-projecting neuron ； Tracer ； Rodent

Zoological Studies

41卷2期（2002 / 04 / 01）

162 - 169

英文

本研究係探討沙鼠舌下神經周核之小腦蚓部投射膽囊激素神經元的分布情形。利用免疫組織化學法可知膽囊激素性神經元主要分布於舌下神經周核的羅勒神經核及舌下神經前置核，而閏核則非常少；且這些膽囊激素性神經元主要為小型神經細胞及部份的中型細胞。進一步利用山葵過氧化氫酶作為神經追蹤劑，多點注射小腦蚓部的皮質，則發現在羅勒核、閏核和舌下神經前置核內有許多膽囊激素性神經元受山葵過氧化氫酶雙重標誌。進一步分析得知在舌下神經周核約有百分之九十的膽囊激素性神經元同時受此追蹤劑所標示。因舌下神經周核內含大量膽囊激素性神經元，且羅勒核和舌下神經前置核可能發出膽囊激素性苔狀神經纖維至小腦蚓部，所以我們推測膽囊激素可作為此投射中神經訊息的重要傳遞或調節物質。

This study aimed to examine the distribution of vermis-projecting cholecystokinin-immunoreactive (CCK-ir) neurons in perihypoglossal (PH) nuclei of the gerbil. Revealed by immunohistochemistry, CCK-ir neurons were mainly distributed in the nucleus of Roller (Ro) and prepositus hypoglossal nucleus (Pr), with fewer cells being located in the nucleus intercalatus (Ic). Small CCK-ir cells made up the majority of cells in the Ro, Ic, and Pr, the remainder being medium-sized. Following multiple injections of horseradish peroxidase (HRP) into the vermal cortex, the majority of CCK-positive neurons in the Ro, Ic, and Pr were labelled. On closer analysis, about 90% of CCK-ir cells in PH nuclei were double labelled. Since the predominant cells in the subnuclei of the PH are CCK-ir, and the subnuclei Ro and Pr are known to give rise to CCK-ir mossy fibers in the vermis of the cerebellum, we conclude that CCK may serve as a putative neurotransmitter or neuromodulator in this projection.