Investigation of Possible Sex Pheromone Components of Female Loreyi Leafworm, Acantholeucania loreyi (Duponchel) (Lepidoptera: Noctuidae) in Taiwan

羅氏夜蛾Acantholeucania loreyi (Duponchel)雌蟲性費洛蒙之探討

賀孝雍(Hsiao-Yung Ho)；蔡如秀(Ru-Shiow Tsai)；徐爾烈(Err-Lieh Hsu)；周延鑫(Yien-Shing Chow)；寇融(Rong Kou)

EAG ； Z7-12:Ac ； Z7-14:Ac ； Z9-14:Ac ； Z7-16:Ac ； Z11-16:Ac ； EAG ； Z7-12:Ac ； Z7-14:Ac ； Z9-14:Ac ； Z7-16:Ac ； Z11-16:Ac

Zoological Studies

41卷2期（2002 / 04 / 01）

188 - 193

英文

羅氏夜蛾雌蟲的腹部末端性費洛蒙腺體內，共鑑定出12:Ac, Z7-12:Ac, 14:Ac, Z7-14:Ac, Z9-14:Ac, 16:Ac, Z7-16:Ac及Z11-16:Ac等八種成分，其中以Z7-12:Ac, Z9-14:Ac及Z11-16:Ac為主成分。由2、3、4及5日齡開始求偶行為的處女雌蟲分析結果顯示，其性費洛蒙腺體內的三個主要成分在不同日齡個體具有廣泛且相似的含量分布(X=121.3±9.4 ng/雌蟲，範圍為66.2~260.5 ng/雌蟲)及比例(Z7-12:Ac/Z9-14:Ac/Z11-16:Ac = 14/72/14，範圍為13-16/70-72/12-17)。Z7-12:Ac, Z7-14:Ac, Z9-14:Ac 及Z7-16:Ac可引起強烈的EAG反應。Z9-14:Ac及Z7-12:Ac (85/15)混合物能引起相當於雌蟲萃取液的EAG反應，而Z11-16:Ac的加入並無引起EAG的加成反應。

In total, 8 components, i.e., dodecyl acetate (12:Ac), (Z)-7-dodecenyl acetate (Z7-12:Ac), tetradecyl acetate (14:Ac), (Z)-7-tetradecenyl acetate (Z7-14:Ac), (Z)-9-tetradecenyl acetate (Z9- 14:Ac), hexadecyl acetate (16:Ac), (Z)-7-hexadecenyl acetate (Z7-16:Ac), and (Z)-11-hexadecenyl acetate (Z11- 16:Ac), were extracted and identified from Acantholeucania loreyi female tips, with Z7-12:Ac, Z9-14:Ac, and Z11- 16:Ac being the 3 major ones. Virgin females initiating calling at 2, 3, 4, or 5 d old, respectively, were analyzed individually for these 3 components via GC. Among different age groups, broad and similar distributions were found both for the titer (X = 121.3 ± 9.4 ng/female, range 66.2~260.5 ng/female) and for the ratio (a combined wash ratio of Z7-12:Ac/Z9-14:Ac/Z11-16:Ac = 14/72/14, range 13-16/70-72/12-17) of the 3 major components. Single components of Z7-12:Ac, Z7-14:Ac, Z9-14:Ac, and Z7-16:Ac elicited strong electroantennogram (EAG) responses in 3-d-old virgin males; the other 4 components, 12:Ac, 14:Ac, 16:Ac, and Z11-16:Ac, elicited little EAG response. A mixture of Z9-14:Ac and Z7-12:Ac (in a ratio of 85/15) elicited an EAG response comparable to that elicited by the female extract, and further addition of Z11-16:Ac to this mixture (in a ratio of 72/14/14) showed no additive effect on the EAG response.