Genotoxicity of Methoxyphosphinyl Insecticide in Mammalian Cells

T.C. Wang；Chih-Min Lin；Li-Wen Lo

SCE ； Chromosome aberration ； Gene mutation ； Organophosphorus insecticide

Zoological Studies

42卷3期（2003 / 07 / 01）

462 - 469

英文

The genotoxicity of 5 organophosphorus insecticides containing the methoxyphosphinyl subunit was assayed by examining the induction of sister-chromatid exchanges (SCEs), chromosome aberrations, and the hypoxanthine-guanine phosphoribosyl transferase (hgprt) gene mutations in CHO cells. Insecticides included acephate, dichlorvos, monocrotophos, methamidophos, and trichlorfon. They consistently induced significant SCEs, with the order of induction potential of acephate ＞ trichlorfon ＞ monocrotophos ＞ methamidophos ＞ dichlorvos. However, only 2 of them, dichlorvos and methamidophos, induced positive chromosome aberrations. Monocrotophos and acephate were questionable positive, while trichlorfon was negative for chromosome aberration induction. The order of chromosome aberration induction potential was dichlorvos ＞ methamidophos ＞ monocrotophos ＞ acephate ＞ trichlorfon. None of these 5 insecticides induced significant hgprt gene mutations compared to the concurrent negative control. The discrepancy between the results of the 2 cytogenetic endpoints, and the contradictory outcome between hgprt mutation and SCE are discussed, from which a possible mechanism of insecticide genotoxicity postulated.