Determination of Cobalamin in Chlorella Food by Cation Exchange Column and Graphite Furnace Atomic Absorption Spectrometry

Shiau-Dune Yang；Yu-Ju Feng；Weiguang Fu

cobalamin ； vitamin B12 ； chlorella food ； graphite furnace atomic absorption spectrometry GFAAS ； method detection limit

藥物食品分析

14卷1期（2006 / 03 / 01）

50 - 53

英文

A method for the rapid determination of cobalamin (Vitamin B12) in chlorella food was proposed. This method utilized a strong cation exchange column to remove free cobalt (Ⅱ), and the cobalamins were quantitatively determined by measuring total cobalt in the column effluent with graphite furnace atomic absorption spectrometry (GFAAS). Under optimized conditions, the method detection limit was 24 μg /100 g. The method was validated by spiking cobalamin standards in different chlorella food, recoveries from spike preparations ranged from 98.3 to 99.5%, with coefficients of variation of 1.2~4.9%. The results show the suitability of the proposed method for determining vitamin B12 in chlorella food.