Arsenic Speciation in Fish on the Market

市售魚肉中不同砷物種之檢測

林浩潭(Haw-Tarn Lin)；陳素文(Su-Wen Chen)；沈季蓉(Chi-Jung Shen)；初建(Chien Chu)

砷物種分析 ； 魚肉 ； 砷酸甜菜鹼 ； 每週攝取量 ； Arsenic speciation ； fish ； arsenobetaine ； weekly intake

藥物食品分析

16卷4期（2008 / 09 / 01）

70 - 75

英文

利用快速蘇氏萃取法及HPLC-ICPMS建立水產品中砷酸甜菜鹼、雙甲基砷、單甲基砷、亞砷酸及砷酸同時檢測方法（回收率分別為：95、111、83、85及84%）。檢測鮪魚肉（BCR627, tuna fish）及鯊魚肉（DORM-2, dogfish muscle）等標準參考樣品中砷酸甜菜鹼之回收率分別爲：95及90%，檢測極限經推估爲0.02 mg/kg。檢測市售60件水產品（頭足類20件、小型魚類16件及大型魚類24件）之結果顯示，水產品中可檢測出54至100%之砷酸甜菜鹼，微量砷酸（平均含量爲0.10 mg/kg），檢測不到雙甲基砷、單甲基砷及亞砷酸。頭足類、小型魚類及大型魚類中砷酸甜菜鹼之含量分別爲5.42、1.57 及1.54 mg/kg。經由取食水產品而攝入砷之安全評估結果，顯示總砷攝取量爲30.6μg/kg body weight/week，高於每人每週容許攝取量15μg/kg body weight/week（無機砷），但其中87%爲對人體無毒之砷酸甜菜鹼，因此尚在安全範圍內。

Arsenic speciation in fish samples on the market was analyzed using gradient anion exchange high performance liquid chromatography (HPLC) with inductively coupled plasma mass spectrometry (ICPMS) detection. Freeze-dried samples were extracted by methanol and water mixture (8:2v/v) with a high-speed Soxhlet system; the concentration of arsenobetaine (AsB), dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), arsenate (As(V)) and arsenite (As(Ⅲ)) in the extract were then determined using HPLC-ICPMS. The method for determining arsenobetaine concentration was confirmed using standard reference material (SRM) of BCR 627 (tuna fish) and DORM-2 (dogfish muscle); recovery rates were 95 and 90%, respectively. The arsenic compounds in 60 market-ready fish muscle samples were investigated. The predominant arsenic compound found in samples was AsB. Arsenate was detected in low concentrations (ranged in 0.02-0.34 mg/kg As for fresh weight), whereas DMAA, MMAA and arsenite content were undetectable. Average AsB content in cephalopods, small fish and large fish were 5.42, 1.57 and 1.54 mg/kg As (fresh weight), respectively. The weekly intake of As was calculated based on the consumption of fish by Taiwanese residents. The calculated results demonstrated that the intake is 30.6 μg/kg (total As) body weight/week, higher than the acceptable weekly intake of 15 μg/kg body weight/week for inorganic arsenic that was suggested by WHO. However, around 87% of As in fish muscle was AsB. AsB is non-toxic and non-carcinogenic to humans, and is rapidly excreted after ingestion. Therefore, intake of fish muscle is low risk based on investigation results.